Effects of Copper Stress on Seed Germination and Physiological Characteristics of Sophora Japonica Seedlings
Under field conditions,5 different concentrations of copper solutions were set up to study the effects of copper stress on the germination of Sophora japonica seeds and physiological characteristics of seedlings.The results showed that the seed germination index,seedling growth index and chlorophyll content of Sophora japonica increased first and then de-creased with the increase of Cu2+concentration.And all the indicators reached their highest levels in T2 treatment.Com-pared with contrast,the O2-content,MDA content,4 protective enzymes activities and proline content in the seedlings of Sophora japonica significantly increased when the concentration of Cu2+concentration exceeded 450 mg/L.When the con-centration of Cu2+concentration exceeded 750 mg/L,the root length,seedling height and fresh weight of Sophora japonica seedlings significantly decreased,while the H2O2 content,soluble protein content and soluble sugar content significantly in-creased.In summary,Cu2+concentration ranging from 0 mg/L to 150 mg/L could promote seed germination and seedling growth of Sophora japonica.However,significant stress effects would occur when Cu2+concentration exceeded 750 mg/L.Therefore,the Cu2+concentration in Sophora japonica seedlings should not exceed 750 mg/L.
Sophora japonicaCopper stressSeed germinationPhysiological characteristics of seedlings
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