Effects of Graphene Oxide on the Tissue Culture Seedlings of Cotinus Coggyria
Using Cotinus coggyria tissue culture seedlings as material,the effect of different concentrations of graphene ox-ide on its primary culture and proliferative culture was studied.The results showed that adding appropriate concentrations of graphene oxide to the primary and proliferation media of Cotinus coggyria tissue culture could reduce the browning rate of tissue culture seedlings and improve plant proliferation index,but the concentration should not be too high.The suitable concentration of oxidized graphene in the primary medium was 1 mg/L～2 mg/L,and oxidized graphene was suitable for use in combination with vitamin C.The appropriate concentration of graphene oxide in the proliferation medium was 0.5 mg/L～1.0 mg/L.The combination of graphene oxide and multiple amino acids could promote the growth and develop-ment of Cotinus coggyria tissue culture seedlings.
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