Prunus x Cistena with apical bud stem segment as explants was chosen in the plant nursery. Results showed that, explants were sterilized with 5% NaCIO adding a droplet TWEEN80 for 20 minutes, which were regarded as the best effectiveness. It was the most suitable for material selection from the middle ten days of March to the middle ten days of April. For the initiation culture, 1/2 MS + 6-BA 1.5 mg/L + NAA 0.05 mg/L was selected as the best combination incubation media recipe. For the rooting culture, the best incubation media was 1/2 MS + IBA 1.0 mg/L + IAA 1.0 mg/L, the better was 1/2 MS + NAA 1.5 mg/L. Root outgrew for 1/2 MS + IBA 1.0 mg/L + IAA 1.0 mg/L in one week, the average root numbers were in the range of 2.0 ~ 5.4 in 20 days, the rate of root was 48.6% to 94.7%.
NETL
NSTL
维普
万方数据
