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火龙果茎段组织培养快繁技术研究

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以火龙果的幼嫩茎段为外植体进行组培快繁研究,结果表明,火龙果不同长度的茎段均能产生愈伤组织,茎上的不定芽大部分可以启动生长,其中,以3cm长的幼嫩茎段诱导效果最佳,愈伤组织诱导率为100%;不定芽诱导培养基以MS+ 6-BA 4 mg/L+ NAA 0.1 mg/L效果最好,诱导率可达75%;当6-BA质量浓度为8mg/L和NAA质量浓度为0.05 mg/L时,不定芽的繁殖系数最高,当6-BA质量浓度为1 mg/L和NAA质量浓度为0.2 mg/L时,虽然不定芽的繁殖系数低,但其不定芽生长较快、苗壮,可以依生产的需要交替使用这2种培养基;适宜的生根培养基为1/2 MS+ IBA 2.0 mg/L.
Tissue Culture and Rapid Propagation of Hylocereus undatus Stem
Tissue culture and rapid propagation of Hylocereus undatus young stem were studied. The results showed: stems of different length could be induced and produced callus, and most adventitious buds could he initiated to grow, in which the best callus inductivity for 3 cm length stem reached 100%; The best culture medium of adventitious buds induction was MS + 6-BA 4.0 mg/L + NAA 0.1 mg/L on which inductivity reached 75%; The propagation ratio of adventitious buds was the highest when concentration of 6-BA was 8 mg/L and NAA 0.0S mg/L, but the growth of adventitious buds was the fastest at concentration of 1 mg/L 6-BA and 0.2 mg/L NAA. So we often use these two mediums alternately according to demands; The best rooting culture medium was 1/2 MS + IBA 2.0 mg/L.

Hylocereus undatustissue culture and rapid propagationcallusinductivitypropagation ratio

王云山、周美虹、康黎芳、曹冬梅、张超、段九菊

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山西省农业科学院园艺研究所,山西太原030031

太原市迎泽李园农业科技培育场,山西太原030045

火龙果 组培快繁 愈伤组织 诱导率 繁殖系数

山西省科技攻关项目山西省科技攻关项目

20080311010-120090312011

2012

山西农业科学
山西省农业科学院

山西农业科学

CSTPCD
影响因子:0.777
ISSN:1002-2481
年,卷(期):2012.40(5)
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