Aimed at rapid reproduction, the experiment was conducted with Carex heterolepis seeds as the materials through sterile culture. The results indicated that: the media containing 1/2 MS + 6-BA 1.0 mg/L + NAA 0.1 mg/L was the most suitable for seed germination; the media containing MS + 6-BA 1.0 mg/L + NAA 0.1 mg/L was the most suitable for the proliferation of seedling subculture; the media containing MS + NAA 0.2 mg/L was the easiest to regenerate root for seedlings.