秋水仙素处理甘草种子染色体加倍研究
Study on Doubling Chomosomes of Licorice Seeds Treated by Colchicine
杨振华1
作者信息
- 1. 杨凌职业技术学院生物工程分院,陕西杨凌712100
- 折叠
摘要
在低温下,用0.05％,0.20％,0.40％秋水仙素水溶液分别处理甘草的萌动种子1,2,3d,干种子4,6,8d,比较不同质量分数处理和不同处理时间对种子的萌发率、加倍率及嵌合率的影响.结果表明,用0.40％秋水仙素处理萌动种子3d染色体加倍效果最好,加倍率可达66.67％;萌发率随处理时间的延长有降低的趋势;嵌合率因秋水仙素质量分数和时间的不同而各异,最高可达34.62％.
Abstract
The dry seeds and germinating seeds of the licorice were treated with 0.05％,0.20％,0.40％ colchicines under low temperature for 4,6,8 d and 1,2,3 d,respectively.To study the influence of different concentration and time of colchicines to the germinating rates,the chromosome-doubled rotes and the embedded rates.The results showed that the highest chromosome-doubled rate was got after germinating seeds were treated with 0.40％ colchicines for 3 d,it was up to 66.67％.With time prolonging,the germinating rates had the trend to decline;the embedded rates were different at different concentration of colchicines and treatment time,the highest rate was up to 34.62％.
关键词
甘草/秋水仙素/萌动种子/干种子/染色体加倍Key words
licorice/colchicin/the germinating seeds/the dry seeds/doubling chromosome引用本文复制引用
基金项目
杨凌职业技术学院校内研究基金(201400020)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据