Screening of Optimal Medium for Callus Inducing and Differentiation of Petiole of Pinellia ternata(Thunb.) Breit
Petioles of Pinellia ternata (Thunb.) Breit were used to investigate the effects of several mediums on the induced dedifferentiation ratio,callus proliferation ratio of petioles of Pine llia ternata (Thunb.) Breit.The results showed that the optimal medium for petioles dedifferentiation,callus proliferation and differentiation were MS + 1.0 mg/L 2,4-D + 0.2 mg/L 6-BA,MS + 1.0 mg/L 2,4-D + 0.2 mg/L 6-BA,MS + 0.5 mg/L 6-BA + 0.2 mg/L NAA.