Screening and Identification of Salt-Tolerant Germplasm of Brassica napus
In order to gain rapeseed(Brassica napus)germplasm suitable for planting in saline alkali land and achieve com-prehensive utilization of the saline soil,in this study,6 rapeseed germplasm materials including Westar,G101,G112,bgr5,ZS11,and G135 were selected from 523 rapeseed germplasms tested by paper bed with 1.5%of NaCl.Systematic evaluation encompassed germination rates and various growth parameters of the materials under agar salt stress and potted salt stress,in-cluding hypocotyl and root lengths,as well as fresh weight and physiological indices(SOD,CAT,proline,etc.).The results showed that G135 exhibited superior performance under saline stress conditions on 1/2 MS medium,displaying enhanced ger-mination rates,elongation of hypocotyls and roots,and superior fresh weight compared to other 5 germplasms.The order of the salt-tolerance ability of the 6 germplasms was G135>Westar>bgr5>G112>ZS11>G101 after comprehensive evalua-tion by membership function.In the pot trials,the growth parameters of G135 were also superior to those of other 5 germ-plasms under the salt stress,and membership function analysis reiterated G135's preeminence in salt tolerance,surpassing G112,Westar,ZS11,G101,and bgr5.Six germplasms were subjected to 0.8%of NaCl stress at the seedling stage in the pot trial,and the antioxidant enzyme activity and proline content of G135 were higher than those of other treatments.After compre-hensive evaluation by membership function,the order of the salt tolerance of 6 germplasms was G135>G112>Westar>bgr5>ZS11>G101.In conclusion,G135,as a salt-tolerant germplasm,showed stable and highly salt-tolerant ability,and could be used for salt-tolerant breeding for rapeseed and study on molecular mechanism of salt-tolerance.
Brassica napuspaper bed germinationsalt-tolerant germplasmmembership function
万方数据
维普
