摘要
为实现"以种适地"的盐碱综合利用的目标,获得适合盐碱地种植的油菜种质,采用1.5%NaCl通过纸床快速筛选法从 523份甘蓝型油菜种质材料中,筛选出不同萌发率的 6份油菜种质材料(Westar、G101、G112、bgr5、ZS11、G135),对其在琼脂盐胁迫和盆栽盐胁迫下的萌发率、下胚轴长、根长、鲜质量及生理指标(SOD、CAT、脯氨酸等)进行测定.结果表明,在1/2 MS培养基盐胁迫下,G135的萌发率、下胚轴长、根长以及鲜质量都优于其他5份材料,采用隶属函数综合评价得出,6份种质的耐盐能力大小为G135>Westar>bgr5>G112>ZS11>G101;在盆栽试验中,从6份种质对不同盐浓度胁迫的响应看,G135的萌发率、下胚轴长、根长以及鲜质量在高盐浓度下均优于其他5份材料,采用隶属函数综合评价得出,6份种质耐盐能力大小为G135>G112>Westar>ZS11>G101>bgr5;在盆栽幼苗期对6份种质进行0.8%NaCl胁迫,G135的抗氧化酶活性和脯氨酸含量高于其他处理,采用隶属函数综合评价得出,6份种质耐盐能力大小为G135>G112>Westar>bgr5>ZS11>G101.综上,G135作为耐盐种质,表现出稳定的高耐盐能力,可用于油菜的耐盐性育种及耐盐分子机理研究.
Abstract
In order to gain rapeseed(Brassica napus)germplasm suitable for planting in saline alkali land and achieve com-prehensive utilization of the saline soil,in this study,6 rapeseed germplasm materials including Westar,G101,G112,bgr5,ZS11,and G135 were selected from 523 rapeseed germplasms tested by paper bed with 1.5%of NaCl.Systematic evaluation encompassed germination rates and various growth parameters of the materials under agar salt stress and potted salt stress,in-cluding hypocotyl and root lengths,as well as fresh weight and physiological indices(SOD,CAT,proline,etc.).The results showed that G135 exhibited superior performance under saline stress conditions on 1/2 MS medium,displaying enhanced ger-mination rates,elongation of hypocotyls and roots,and superior fresh weight compared to other 5 germplasms.The order of the salt-tolerance ability of the 6 germplasms was G135>Westar>bgr5>G112>ZS11>G101 after comprehensive evalua-tion by membership function.In the pot trials,the growth parameters of G135 were also superior to those of other 5 germ-plasms under the salt stress,and membership function analysis reiterated G135's preeminence in salt tolerance,surpassing G112,Westar,ZS11,G101,and bgr5.Six germplasms were subjected to 0.8%of NaCl stress at the seedling stage in the pot trial,and the antioxidant enzyme activity and proline content of G135 were higher than those of other treatments.After compre-hensive evaluation by membership function,the order of the salt tolerance of 6 germplasms was G135>G112>Westar>bgr5>ZS11>G101.In conclusion,G135,as a salt-tolerant germplasm,showed stable and highly salt-tolerant ability,and could be used for salt-tolerant breeding for rapeseed and study on molecular mechanism of salt-tolerance.
国家科技期刊平台
NSTL
维普
万方数据