Identification of GARP Transcription Factor Family and Cloning and Expression of Bulb Development Related Genes in Lilium
GARP is a plant specific transcription factor family and plays a key role in plant growth,development,and abiotic stress response.In order to provide basic information for a comprehensive understanding of GARP transcription factors in Lilium,and laid a foundation for exploring the growth and development mechanism of bulbs in Lilium,in this study,based on the transcriptome data of Next-generation sequencing and Third-generation sequencing of Lilium,identification and expression analysis of GARP family members were conducted.The results indicated that a total of 34 GARP proteins were identified in transcriptome data,all the GARP proteins were hydrophilic,68%of which were acidic proteins,and 94%were unstable proteins.Based on the phylogenetic analysis,Lilium GARPs were clustered into five subfamilies:ARR-B,GLK,NIGT1/HRS1/HHO,PHR1/PHL1,and KAN,and they were annotated with functions on growth and development,biological clock regulation,flowering transformation,hormone transport,abiotic stress and other processes.The ARR-B subfamily members that played important roles in bulb development were analyzed by qRT-PCR,and the results showed that LhGARP33,LhGARP30,and LhGARP3 may play important roles in bulb development.The LhGARP3 and LhGARP30 were successfully cloned and their amino acid sequences exhibited the typical ARR-Bs phosphate receptor domain(REC)and metal and phosphorylation binding sites.Spatial expression patterns of ARR-B I subgroup in flowers,leaves,and bulbs were analyzed and the results revealed that LhGARP5 had the highest expression in leaves,while LhGARP31 had the highest expression in flowers,LhGARP33,LhGARP30 and LhGARP3 had the basically consistent expression characteristics,the highest expression in bulbs,indicating that they may play a major role in bulb development as a key gene.
LiliumGARP familybulb developmentreal time fluorescence quantitative PCRgene cloning
万方数据
维普
