首页|不同授粉方式下砀山酥梨早期受精生理特性与授粉效果分析

不同授粉方式下砀山酥梨早期受精生理特性与授粉效果分析

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为探究不同授粉方式对砀山酥梨(Pyrus bretschneideri cv.Dangshansu)早期受精生理特性、果实动态发育和果实品质的影响,以人工授粉为对照、蜜蜂授粉为处理组,使用显微镜观察人工授粉和蜜蜂授粉后梨花花粉萌发差异,采用石蜡包埋子房切片观察子房发育差异,结合果实发育动态监测与果实品质检测,揭示2种授粉方式对梨树果实生长发育的影响.结果表明,相较于人工授粉,蜜蜂授粉后梨花花粉萌发较早,授粉后2 h已在柱头萌发,授粉后48 h到达花柱基部,椭圆型胚囊完整性更好.子房发育切片观察表明,相较于人工授粉,蜜蜂授粉后胚珠发育更快,且后期胚珠发育为种子数量更多.人工授粉和蜜蜂授粉的花序和花朵坐果率均可达到生产需要;蜜蜂授粉后,果实中苹果酸、柠檬酸和奎尼酸含量均极显著降低,葡萄糖、蔗糖和山梨醇含量均极显著升高,总酚含量显著升高.利用蜜蜂为梨树授粉,花粉萌发和到达子房基部的时间均早于人工授粉,还可以显著提升梨的果实品质.
Analysis of Physiological Characteristics of Early Fertilization and Pollination Effects of Pear(Pyrus bretschneideri cv.Dangshansu)under Different Pollination Methods
To explore the effects of different pollination methods on the physiological characteristics of early fertilization,fruit dynamic development,and fruit quality of pear(Pyrus bretschneideri cv.Dangshansu),in this study,artificial pollination was used as the control group,and honeybee pollination was used as the treatment group,a microscope was used to observe the differences in pollen germination between pear flowers after artificial pollination and honeybee pollination,paraffin embedded ovary slices was to used to observe differences in ovary development,combined with the dynamic monitoring of fruit development and quality detection,the effect of the two pollination methods on the growth and development of pear fruit was revealed.The results showed that compared with artificial pollination,pear flower pollen germinated earlier after honeybee pollination,germinated at the stigma 2 hours after pollination and reached the base of the stigma 48 hours after pollination.The integrity of oval embryo sac was better.The observation of ovary development slices showed that compared with artificial pollination,the ovules developed faster after honeybee pollination,and the ovules developed into more seeds in the later stage.The inflorescence and flower fruit setting rate of artificial pollination and honeybee pollination could achieve the production needs.After honeybee pollination,the contents of malic acid,citric acid,and quinic acid decreased significantly,the contents of glucose,sucrose,and sorbitol increased significantly,and the content of total phenols increased significantly.By using honeybees to pollinate pear trees,pollen germinated and reached the base of the ovary earlier than artificial pollination,it could also significantly improve the fruit quality of pears.

Pyrus bretschneideri cv.Dangshansuhoneybee pollinationartificial pollinationfertilization physiologyfruit quality

张旭凤、王锋、曹嵌、武敏、郭宝贝、郭媛

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山西农业大学 园艺学院,山西 太原 030031

山西农业大学 动物科学学院,山西 晋中 030801

山西农业大学 果树研究所,山西 晋中 030815

砀山酥梨 蜜蜂授粉 人工授粉 受精生理 果实品质

2025

山西农业科学
山西省农业科学院

山西农业科学

影响因子:0.777
ISSN:1002-2481
年,卷(期):2025.53(1)