成年鸭睾丸发育差异分析
Differential Analysis of Testicular Development in Adult Ducks
陶志云 1宋卫涛 1徐文娟 1朱春红 1刘宏祥 1王志成 1顾昊天 1王逸飞 1李慧芳1
作者信息
- 1. 江苏省家禽科学研究所,江苏 扬州 225125
- 折叠
摘要
为探讨不同精液量鸭的睾丸发育差异,以成年高邮鸭为研究对象,根据个体精液量体积分为精液量多和精液量少组,分别称量2组鸭的体质量和睾丸总质量;采用ELISA法检测2组血液中的睾酮含量;对2组鸭睾丸组织石蜡切片后显微观察并测量睾丸曲精小管面积、单位面积间质细胞数量,比较2组间的差异,并进行相关性分析;最后利用荧光定量PCR技术检测2组鸭睾丸组织中FSHR、LHR、AMH、StAR的表达水平.结果表明,精液量多组的睾丸总质量、睾丸体积和睾酮含量显著高于精液量少组的睾丸总质量、睾丸体积和睾酮含量;体质量、睾丸指数、曲精小管面积和单位面积间质细胞数量在2组间无显著差异.相关性分析结果表明,精液量与睾丸体积呈显著正相关,睾酮含量与睾丸总质量和睾丸体积呈显著正相关.精液量多组中睾丸发育相关基因FSHR、LHR、AMH、StAR的mRNA表达水平均显著高于精液量少组.综上所述,公鸭的精液量多少与睾丸总质量和睾酮含量有关,而且睾丸发育相关基因FSHR、LHR、AMH、StAR在鸭睾丸发育和精子生成过程中可能发挥了重要作用.
Abstract
In order to explore the differences in testicular development in ducks with different semen volumes,in this study,adult Gaoyou ducks were taken as the research object.These ducks were divided into two groups:high semen volume and low semen volume groups.The weight and testicular weight of the two groups of ducks were measured,respectively.ELISA method was used to detect testosterone levels in the blood of the two groups.Microscopic observation and measurement of the area of the seminiferous tubules and the number of interstitial cells per unit area were performed after paraffin sectioning on the two groups of duck testicular tissue.The differences between the two groups were compared and correlation analysis was conducted.Finally,fluorescence quantitative PCR was used to detect the expression levels of FSHR,LHR,AMH,and StAR in the two groups of duck testicular tissues.The results showed that the testicular weight,testicular volume,and testosterone levels in the high semen volume group were significantly higher than those in the low semen volume group.There was no significant difference in body weight,testicular index,seminiferous tubule area,and number of interstitial cells per unit field between the two groups.Correlation analysis showed that semen volume was significantly positively correlated with testicular volume.While testosterone content was significantly positively correlated with testicular weight and volume.The expression levels of testicular development related genes FSHR,LHR,AMH,and StAR.were significantly higher in the high semen volume group than those in the low semen volume group.These results indicated that the semen volume in male ducks was related to testicular weight and testosterone content.And testicular development related genes FSHR,LHR,AMH and StAR may play important roles in duck testicular development and spermatogenesis.
关键词
高邮鸭/睾丸/发育/ELISA法/相关性分析Key words
Gaoyou ducks/testis/development/ELISA method/correlation analysis引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
维普
万方数据