Inhibitory Effect of Soluble Dietary Fiber from Foxtail Millet on Colon Cancer Cell Line HT-29
In order to explore the effect and mechanisms of soluble dietary fiber from foxtail millet(FMB-SDF)on growth and apoptosis of colon cancer cell line HT-29,in this study,using different mass concentrations(2.5,5,7.5 and 10 mg/mL)of FMB-SDF as experimental groups,the effect of FMB-SDF on the proliferation of RAW264.7 macrophages in mice was de-tected by MTT assay.The levels of superoxide dismutase(SOD)activity and lactate dehydrogenase(LDH)activity were de-tected using a reagent kit method.Secretion of tumor necrosis factor(TNF-α),cytokines interleukin-6(IL-6),and interleukin-10(IL-10)were detected by ELISA in colon cancer cells HT-29.At the same time,Rhodamine 123 probe was used to detect the mitochondrial membrane potential changes of colon cancer cell HT-29.The results showed that FMB-SDF was able to in-hibit the growth of colon cancer cells HT-29 by interfering with the redox system.Meanwhile,FMB-SDF scaled up the secre-tion of anti-inflammatory factor IL-10 by inhibiting the secretion of inflammatory factors IL-6 and TNF-α,which in turn re-strained the development of inflammation in colon cancer cells.Further experimental results showed that FMB-SDF was able to enhance the mitochondrial membrane potential damage in HT-29 cells.In conclusion,these results indicated that FMB-SDF induced apoptosis in HT-29 cells by interfering with the redox system,inhibiting the development of inflammation,and promoting mitochondrial membrane potential damage.
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