Optimization Extraction,Separation and Purification Technology of Total Flavonoids from Prinsepia utilis Royle
Prinsepia utilis Royle is a characteristic biological resource in northwest Yunnan province,Prinsepia utilis Royle contains flavonoids,but the extraction rate and purity were low,and the research is limited.In order to extract and purify total flavonoids from Prinsepia utilis Royle efficiently,in this study,the extraction rate of Prinsepia utilis Royle total flavonoids was used as the evaluation index,and orthogonal design was used to optimize the extraction parameters of Prinsepia utilis Royle total flavonoids by ultrasonic assistance,the ideal resin and purification conditions for purifying Prinsepia utilis Royle total flavonoids were selected,the total flavonoids of Prinsepia utilis Royle were further purified by large-pore resin combined with polyamide column chromatography,and the purity of Prinsepia utilis Royle total flavonoids was calculated.The results showed that the optimal extraction method for Prinsepia utilis Royle total flavonoids by ultrasonic assistance was as follows:ethanol volume fraction of 60%,solids-to-liquid ratio of 1:60 g/mL,and extraction time of 70 min.Under these conditions,the extraction rate of Prinsepia utilis Royle total flavonoids was 7.97%.The factors that had the greatest impact on the extraction rate of Prinsepia utilis Royle total flavonoids in order of importance were ethanol volume fraction>solids-to-liquid ratio>extraction time.The ideal resin for separating and purifying Prinsepia utilis Royle total flavonoids was HPD-500 resin.By drawing static adsorption/desorption curves,the static adsorption saturation time was obtained and was 240 min,and the static desorption equilibrium time was 120 min.The optimal purification process parameters were as follows:pH of the feed solution was 4,feed solution concentration was 1.30 mg/mL,eluent volume was 4 BV,eluent was 60%of ethanol.Under these conditions,the purity of Prinsepia utilis Royle total flavonoids was 33.19%.After purification by large-pore resin combined with polyamide,the purity of Prinsepia utilis Royle total flavonoids was 62.48%,with an increase of 29.29%over the purification by large-pore resin and an increase of 54.51%over the raw material before purification.
Prinsepia utilis Royletotal flavoneultrasonic assisted extraction methodlarge-pore resin combined with polyamide column chromatographyseparation and purification
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