Correlation analysis of serum miR-499 and severity of coronary lesions in patients with acute coronary syndrome
Objective·To explore the value of miR-499 expression levels in the serum of patients with acute coronary syndrome(ACS)in predicting the severity of coronary artery disease.Methods·Seventy-four patients with ACS admitted to Xinhua Hospital,Shanghai Jiao Tong University School of Medicine were included and divided into a mild group of 30 cases,a moderate group of 21 cases,and a severe group of 23 cases according to the Gensini score.Real-time quantitative polymerase chain reaction(qPCR)technology was used to detect the expression level of serum miR-499,while troponin(cTnI),creatine kinase isoenzyme(CK-MB),myoglobin(Mb),brain natriuretic peptide(BNP),left ventricular ejection fraction(LVEF),and other laboratory indicators were measured.Spearman correlation analysis was used to analyze the correlation between the expression level of miR-499 and Gensini score,as well as the number of vessel branches in coronary artery disease.The evaluation value of miR-499 in assessing the severity of coronary artery disease was analyzed by using receiver operating characteristic(ROC)curve.Results·The expression level of miR-499 increased with the severity of coronary artery disease(P=0.000).The expression level of miR-499 was positively correlated with Gensini score and cTnI(r=0.331,r=0.143,P＜0.05),respectively,but not with LVEF(P＞0.05),while Gensini score was not correlated with cTnI and LVEF(P＞0.05).ROC analysis showed that the AUC of miR-499 for assessing the severity of coronary artery disease was 0.802(95％CI 0.698-0.906),with sensitivity of 65.2％and specificity of 80.4％.The expression level of miR-499 increased with the increase of the number of coronary artery lesions,and was positively correlated with the number of coronary artery lesions(r=0.368,P＜0.05).Conclusion·The expression level of miR-499 in the serum of ACS patients is correlated with the severity and number of coronary artery lesions,which has a certain evaluation value in evaluating the severity of coronary artery lesions.
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