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湿地极危植物中华水韭孢子育苗及幼孢苗管护

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采用土壤和改良的Knop’s固体培养基培养湿地敏感植物、国家一级保护极危蕨类中华水韭(Isoetes sinensis),并获得有性生殖苗;观察记录了配子体发育及幼孢苗发生各阶段的详细过程;总结了幼孢苗的人工管护技术;分析讨论了配子体发育与环境因素间的规律、幼孢苗复壮对策以及病虫害类别、病症及其防治措施等.研究结果表明,中华水韭孢子在22~28℃、光照强度为60 μmol/(m2·s)、每日光照14h和黑暗10h的条件下,可达到90.3%的成熟率;已完成中华水韭的生活史,掌握了孢子育苗及日常管护方法,获得孢苗5 000棵,长势良好,出现孢子囊,以期为回归原产地,拯救该野生种群及湿地保护提供一定的理论依据和可靠的技术支撑.
Spore Seeding Cultivation and Care of Young Sporophyte Seedling of Extreme Endangerous Wetland Plant Isoetes sinensis
This paper cultured Isoetes sinensis which is national extreme endangered ferns,using soil and knop's substrate,sexual reproduction seedlings were obtained.The detailed process of every stage of young spore seedling happening and the gametophyte development were observed and recorded.The paper summarized artificial management technology of the young spore seedling.The regulation between the gametophyte development and environmental factors was analyzed,rejuvenation countermeasures of young spore seedling were defined,and category and control measures of pests were discussed.The results showed that the maturation rate of the Isoetes sinensis can reach 90.3%,under the condition of between 22 ℃ and 28 ℃,the light intensity 60 μmol/(m2· s),14 hours light and 10 hours dark every day.The paper had completed the life history of Isoetes sinensis,and grasped the seedling and daily management method and had got 5 000 plants.They grew well and appeared the sporangium,in order to return to the country of origin,and provide certain theoretical basis and reliable technical support of wild population and wetland protection.

extreme endangerous wetland plantIsoetes sinensisspore seedling cultivationbreed young sporophyte seedling

邢建娇、路靖、李范、刘保东

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哈尔滨师范大学,黑龙江省普通高等学校植物生物学重点实验室,黑龙江哈尔滨150025

湿地极危植物 中华水韭 孢子育苗 幼孢苗管护

国家自然科学基金

31170294

2013

湿地科学
中国科学院 东北地理与农业生态研究所

湿地科学

CSTPCDCSCD北大核心
影响因子:1.52
ISSN:1672-5948
年,卷(期):2013.11(3)
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