小鼠胃癌模型Nesfatin-1水平及其在胃癌共病抑郁发病中的作用
The Level of Nesfatin-1 in a Mouse Gastric Cancer Model and Its Role in Gastric Cancer Comorbid with Depression
张楠 1李江波 2王惠玲 1肖玲 1魏艳艳 1何静 1王高华1
作者信息
- 1. Mental Health Center of Wuhan University Renmin Hospital, Hubei, Wuhan, China
- 2. Clinical Psychology Department of the Second People's Hospital of Wuhu, Anhui, Wuhu, China
- 折叠
摘要
背景:癌症患者伴有抑郁症状的发生率较高,而抑郁情绪又会通过神经-内分泌-免疫网络系统来影响癌症的发生、发展及转归.目的:探讨小鼠胃癌模型血浆及脑组织中Nesfatin-1水平及其在胃癌共病抑郁发病中的作用机制.方法:将18只615小鼠随机分为正常对照组(NCG)、胃癌不合并应激抑郁模型组(GCNS)、胃癌合并应激抑郁模型组(GCs).GCNS组小鼠饲养5周后于皮下接种小鼠前胃癌细胞(MFC)建立皮下移植瘤模型,GCS组给以5周慢性不可预见应激(CUMS)后,皮下接种MFC建立皮下移植瘤模型1周.用旷场实验、蔗糖偏好试验、强迫游泳试验(FST)评价小鼠的行为.分别用酶联免疫吸附法(ELISA)及Western Blot技术来检测血浆及脑组织中Nesfatin-1浓度.结果:GCS组体重增量加显著低于GCNS组(t=-3.39,p＜ 0.001)、且GCS、GCNS两组均低于NCG组(t=-6.33,p＜ 0.001;t =-2.94,p=0.01);旷场试验中GCS组水平移动距离小于GCNS组(f=-2.50,p=0.025),且GCS、GCNS两组二者均小于NCG组(t=-5.87,p＜0.001;t=-3.38,p=0.004);GCS组不动时间长于GCNS组与NCG组(t=2.56,p=0.022;t=3.84,p=0.002).中脑、海马及血浆Nesfatin-1水平NCG组与GCS组均显著高于GCNS组,且而GCS组Nesfatin-1浓度显著高于正常对照组,结果均有统计学意义.结论:胃癌不合并应激抑郁小鼠脑组织及血浆Nesfatin-1水平降低,CUMS应激可以引起胃癌小鼠的抑郁样行为,且可以升高脑组织及血浆Nesfatin-1水平,因此Nesfatin-1可能与胃癌及胃癌相关应激抑郁的发病有关.
Abstract
Background:The incidence of depressive symptoms is higher in cancer patients.And depression can also affect the occurrence,development and outcome of cancer through the neuroendocrine-immunenetwork system.Objective:To study the level of Nesfatin-1 in the plasma and brain tissue and its role in the pathogenesis in gastric cancer comorbid with depression using a mouse gastric cancer model.Methods:18 mice were randomly divided into the normal control group (NCG),gastric cancer without stress model group (GCNS),and gastric cancer combined with stress model group (GCS).The mice of the GCNS group were inoculated subcutaneously with mouse forestomach carcinoma (MFC) after 5 weeks of nomal feeding to establish a model of subcutaneous transplantation tumor.After 5 weeks of chronic unpredicted mild stress (CUMS) in the GCS group,subcutaneous inoculation of MFC was used to establish a subcutaneous transplantation tumor model for 1 week.Evaluation of mice behavior was performed by open field test,sucrose preference test and forced swimming test (FST).Determination of Nesfatin-1 concentration in plasma and brain tissue was carried out using enzyme linked immunosorbent assay (ELISA) and Western Blot.Results:The weight increment in the GCS group was significantly lower than that in the GCNS group (t=-3.39,p＜0.001).And both GCS and GCNS were lower than the NCG group (t=-6.33,p＜0.001;t=-2.94,p=0.01).In the open field test,the horizontal moving distance of the GCS group was less than that of the GCNS group (t=-2.50,p=0.025),and both GCS and GCNS were smaller than the NCG group (t=-5.87,p＜0.001;t=-3.38,p=0.004).The dead time of the GCS group was longer than that of the GCNS and the NCG groups (t=2.56,p=0.022;t=3.84,p=0.002).The Nesfatin-1 level in the middle brain,hippocampus and plasma was significantly higher in NCG group and GCS group than in the GCNS group.The concentration of Nesfatin-1 in the GCS group was significantly higher than that in the NCG group.Conclusions:There is a decrease of Nesfatin-1 level in brain tissue and plasma in mice with gastric cancer without stress.CUMS stress can induce depressive behavior in gastric cancer mice,and increase the level of Nesfatin-1 in brain tissue and plasma.Therefore,Nesfatin-1 may be related to the pathogenesis of gastric cancer stress related depression.
关键词
Nesfatin-1/胃癌/抑郁/应激Key words
Nesfatin-1/gastric cancer/depression/stress引用本文复制引用
基金项目
Funding provided by the Natural Science Foundation of China(81571325)
出版年
2018
NETL
NSTL
万方数据