PURPOSE:To study the structural characteristics of oral microorganisms in children with caries by 16S rRNA high-throughput sequencing technology.METHODS:Thirty healthy children aged 3-5 years were enrolled as subjects.According to the index of dmfs,they were divided into caries-free(CF)group(15)and early childhood caries(ECC)group(15).To compare the differences in bacterial community structure,samples of saliva and dental plaque were collected,and high-throughput sequencing was conducted using the Illumina Miseq sequencing platform.Bioinformatics analysis was used to analyze the difference of microbial community structure and diversity with SPSS 23.0 software package.RESULTS:Mi-crobial diversity in ECC group was significantly lower than CF group.At phylum level,Actinobateria was more abundant in saliva samples of ECC group,while Firmicutes was more abundant in plaque samples of CF group.At genus level,the abun-dance of Lautropia of CF group was higher in saliva samples while Cardiobacterium,Gemella and Granulicatella were abun-dant in plaque samples.The abundance of Rothia of ECC group was higher in saliva samples and Corynebacterium was abundant of ECC group in plaque samples.CONCLUSIONS:There are significant differences in the species and composi-tion of microbial community in saliva and plaque of children with or without caries.Specific microorganisms are related to the occurrence of ECC,and screening specific microorganisms is helpful for early prediction and prevention of ECC.
Early childhood cariesDental plaqueSalivaHigh throughput sequencing
NETL
NSTL
万方数据
