目的:探讨前列腺素E2(prostaglandin E2,PGE2)、环氧合酶2(cyclooxygenase-2,COX-2)表达与牙槽骨成骨活性、内氧水平的关系.方法:选择2021年3月—2023年3月收治的56例慢性牙周炎且牙槽骨感染的患者为试验(牙周炎)组,同一时段53例健康牙槽骨为对照组.采用组织块培养法进行成骨细胞培养,采用改良Kaplow碱性磷酸酶(alkaline phosphatase,ALP)染色鉴定细胞,利用ELISA试剂盒检测COX-2、PGE2、破骨细胞抑制因子(osteoclastogen-esis inhibitory factor,OPG)和核因子 κB 受体活化因子配体(receptor activator of nuclear factor-K b ligand,RANKL)等的表达,比较2组患者PGE2、COX-2、OPG、内氧水平、ALP和RANKL的表达水平,并分析其相关性.采用SPSS 27.0软件包对数据进行统计学分析.结果:牙周炎组的PGE2、COX-2、RANKL显著高于对照组,而OPG、内氧水平、ALP显著低于对照组(P＜0.05).PGE2、COX2呈高度正相关,与OPG、内氧水平和ALP呈高度负相关,但与RANKL呈高度正相关(P＜0.05).结论:PGE2、COX-2表达与ALP、内氧水平呈高度负相关,可考虑通过增加内氧水平,加大氧分压,并通过药物调节ALP水平,改变牙周炎或其他类似疾病的炎症状况.
The relationship between the expression of PGE2 and COX-2 and the osteogenic activity and oxygen level of alveolar bone
PURPOSE:To study the relationship between the expression of prostaglandin E2(PGE2)and cyclooxygenase-2(COX-2)and the osteogenic activity and oxygen level of alveolar bone.METHODS:The alveolar bones of 56 patients with chronic periodontitis who received dental treatment from March 2021 to March 2023 were collected as the experimen-tal(periodontitis)group,and the healthy alveolar bones of 53 patients who received dental treatment during the same peri-od were selected as the control group.The osteoblasts were cultured by tissue block culture,and modified Kaplow's alka-line phosphatase(ALP)staining was used to identify the cells.COX-2,PGE2 and osteoclastogenesis inhibitory factor(OPG)receptor activator of nuclear factor-Kb ligand(RANKL)and other indicators were determined by ELISA.PGE2,COX-2,OPG,internal oxygen level,ALP,RANKL and their correlation were compared between the two groups.Statistical analysis was performed with SPSS 27.0 software package.RESULTS:PGE2,COX-2 and RANKL in periodontitis group were significantly higher than those in the control group,but OPG,internal oxygen level and ALP were significantly lower than those in the control group(P＜0.05).PGE2 and COX2 were highly positively correlated with OPG,internal oxygen level and ALP,but were highly positively correlated with RANKL(P＜0.05).CONCLUSIONS:The expression of PGE2 and COX-2 is highly negatively correlated with ALP and oxygen levels.Clinical treatment may consider increasing oxygen levels,increasing oxygen partial pressure,and regulating ALP levels by drugs,so as to change the inflammatory condition of periodontitis or other dental diseases.
PGE2COX-2Alveolar bone osteogenic activityInternal oxygen levelRelationship
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