摘要
目的:探讨槐角提取物对雌激素缺乏性骨质疏松小鼠牙槽骨骨量的影响.方法:6周龄雌性未孕野生型C57BU6J小鼠,随机分为假手术组、卵巢切除(ovariectomy,OVX)组和OVX+槐角提取物组.OVX组和OVX+槐角提取物组小鼠摘除双侧卵巢,按照每次150 mg/kg剂量槐角提取物对OVX+槐角提取物组小鼠进行灌胃处理,每周3次,连续给药4周,其余2组小鼠同期给予等体积生理盐水灌胃.每周测量体重3次,4周后以micro-CT检测小鼠牙槽骨骨微参数.采用GraphPad Prism 9软件对数据进行处理分析.结果:与假手术组相比,OVX组小鼠牙槽骨术后1个月骨小梁相关参数显著下降(P＜0.05).槐角提取物干预1个月后,雌激素缺乏小鼠牙槽骨骨密度(bone mineral density,BMD)、骨小梁数量(trabecular number,Tb.N)和骨小梁离散度(trabecular separation,Tb.Sp)明显恢复,与假手术组无显著差异(P＞0.05);但骨小梁面积体积比(bone volume fraction,BV/TV)和骨小梁宽度(trabecular thickness,Tb.Th)尚未完全恢复到假手术组水平(P＜0.05).结论:槐角提取物能有效增加雌激素缺乏而减小的牙槽骨骨量,可作为治疗绝经性牙槽骨骨质疏松症的潜在药物之一.
Abstract
PURPOSE:To investigate the effect of Sophora japonica extract on alveolar bone mass in ovariectomized os-teoporosis mice.METHODS:Six-week-old female non-pregnant wild-type C57BU6J mice were randomly divided into sham operation group,ovariectomy(OVX)group and OVX+Sophora japonica extract group.Ovaries of the mice in the OVX group and the OVX+Sophora japonica extract group were removed,and the mice in the OVX+Sophora japonica extract group were treated by Sophora japonica extract at a dose of 150 mg/kg,three times a week for 4 weeks;while mice of the other two groups were given an equal volume of normal saline at the same time.Body weight was measured 3 times a week,and the micro-parameters of alveolar bone were detected by Micro-CT after 4 weeks.The data were analyzed by GraphPad Prism 9 software.RESULTS:Compared with the sham-operated group,the trabecular bone parameters of the alveolar bone in the OVX group were significantly decreased 1 month after operation(P＜0.05).One month after interven-tion with Sophora japonica extract,alveolar bone mineral density(BMD),trabecular number(Tb.N)and trabecular separa-tion(Tb.Sp)in OVX mice was significantly rescued,with no significant difference compared to the sham surgery group(P＞0.05);but bone volume fraction(BV/TV)and trabecular thickness(Tb.Th)had not completely recovered to the levels of the sham-op-erated group(P＜0.05).CONCLUSIONS:Sophora japonica extract can effectively increase the alveolar bone mass reduced by estrogen deficiency and may be used as one of the potential drugs for the treatment of menopausal alveolar bone osteoporosis.
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