摘要
目的:分析龋病患儿唾液离子浓度、链球菌、双歧杆菌变化与病情的关联性.方法:选择2022年5月-2023年4月收治的80例3～6岁龋病患儿为试验组,根据龋失补指数(DMFT)分为轻度组(DMFT≤15％,n=35)和重度组(DMFT＞15％,n=45);另选择进行健康检查的80例3～6岁无龋病患儿为对照组.采用甲基麝香酚蓝检测唾液中钙离子浓度,采用实时定量PCR技术检测链球菌、双歧杆菌水平,采用Pearson分析DMFT与唾液钙离子浓度、链球菌、双歧杆菌的相关性,并采用受试者工作特征曲线(receiver operating characteristic curve,ROC)评估唾液钙离子浓度、链球菌、双歧杆菌对龋病患儿病情严重程度的诊断价值,计算曲线下面积(area under the curve,AUC).采用SPSS 22.0软件包对数据进行统计学分析.结果:试验组唾液钙离子浓度、变形链球菌、远缘链球菌和双歧杆菌水平显著高于对照组(P＜0.05);重度组显著高于轻度组(P＜0.05);DMFT与唾液钙离子浓度、链球菌、双歧杆菌呈正相关(P＜0.05).根据DMFT制作ROC曲线,唾液钙离子浓度、变形链球菌、远缘链球菌和双歧杆菌对评估龋病患儿病情严重程度的AUC分别为0.760、0.658、0.718和0.705.结论:唾液离子浓度、变形链球菌、远缘链球菌和双歧杆菌水平增加与患儿龋病有关,与龋病病情程度呈正相关,对评估病情严重程度有一定价值.
Abstract
PURPOSE:To analyze the correlation between the changes in salivary ion concentration,Streptococcus and Bifidobacterium in children with dental caries and the severity of the disease.METHODS:Eighty children with dental caries treated from May 2022 to April 2023 were selected as the experimental group.According to the DMFT,they were divided into mild group(DMFT≤ 15％,n=35)and severe group(DMFT＞15％,n=45).Another 80 children without dental caries were selected as the control group.The calcium ion concentration in saliva was detected by methanol-permeabi-lized calciometric method,the level of Streptococcus and Bifidobacterium was detected by real-time single-quantity PCR technology,and the correlation between DMFT and salivary calcium ion concentration,Streptococcus and Bifidobacterium was analyzed by Pearson correlation analysis.The diagnostic value of salivary calcium ion concentration,Streptococcus and Bifidobacterium for the severity of dental caries in children was evaluated by the receiver operating characteristic curve(ROC),and the area under the curve(AUC)was calculated.SPSS 22.0 software package was used for statistical anal-ysis.RESULTS:The level of salivary calcium ion concentration,Streptococcus mutans,Streptococcus sobrinus and Bifi-dobacterium in the experimental group was significantly higher than that in the control group(P＜0.05),and significantly higher in the severe group than in the mild group(P＜0.05).DMFT was significantly positively correlated with salivary calci-um ion concentration,Streptococcus and Bifidobacterium(P＜0.05).Based on DMFT,ROC curve was prepared,and AUCs of salivary calcium ion concentration,Streptococcus mutans,Streptococcus sobrinus and Bifidobacterium for assessing the severity of dental caries in children were 0.760,0.658,0.718 and 0.705,respectively.CONCLUSIONS:Increased sali-vary ion concentration,Streptococcus sobrinus,Streptococcus mutans and Bifidobacterium levels are related to dental caries in children,and are positively correlated with the severity of the disease,which has a certain value for assessing the severity of the disease.
基金项目
安徽省自然科学基金青年项目(2108085QH334)
NETL
NSTL
万方数据