Enzymatic characteristics and inhibitions effect of polyphenol oxidase from celery
With UV spectrophotometric method and celery as the experimental material,the aim of this experiment was to research the effect of the enzyme characteristics and inhibitors on the activity of polyphenol oxidase from celery.The results showed that the most suitable temperature was 36 ℃C,and the optimum pH for celery PPO activity was 5.0.Inhibitors tests found that ascorbic acid,L-cysteine,citric acid and calcium chloride could inhibit PPO.The most effective inhibitor was L-cysteine,10 mmol/L completely inhibited the activity of PPO from celery.