Transcriptomic analysis reveals the effect of cold stress on the totipotency of barley microspores
To investigate the effects of low temperature on the pluripotency of microspore cells,barley microspores treated with low temperature for 0,3,and 7 days were cultured in vitro.The results showed that the yield of MDEC increased significantly depending upon cold treatment time.Transcriptome analysis showed that 5 290 and 7 103 differentially expressed genes were obtained at 3 days post treatment(dpt)and 7 dpt when compared with 0 dpt,respectively.GO enrichment analysis showed that the most significant categories in the biological processes at 3 dpt and 7 dpt were carbohydrate metabolism,glutathione metabolic,and sporopollenin biosynthetic process.Nucleosomes,thylakoids,cell wall and other cellular components,as well as DNA binding transcription factor activity were most significant categories in the molecular function.KEGG enrichment analysis showed that the pathways at 3 dpt and 7 dpt were enriched in photosynthesis-antenna proteins,hormone signal transduction,glyoxylate and dicarboxylate metabolism,etc.Plant hormone signal transduction pathway was the most significant among the up-regulated pathways,and the related genes might play important roles in the microspore totipotency.In addition,heat shock protein and glutathione S-transferase,which were related to plant defense pathway,might also be involved in the process of microspore reprogramming.
万方数据
维普
