Establishment of open sugar free tissue culture technology system of blueberry
The main technical aspects of sugar-free culture,such as the type and concentration of bacteriostatic agent,the concentration of liquid medium and the ratio of it to the matrix,and CO2 concentration,were carried out by using blueberry sterile seedlings as materials and L9(34)orthogonal experimental methods.The results showed that(1)When 8 mL/L of bacteriostatic agent"Qingjunyi"was added to the matrix culture medium,and the content of the culture medium in the substrate was 80％,the contamination rate could be controlled within 15％,the growth and proliferation of blueberry seedlings were normal.(2)Orthogonal experimental results ANOVA and range analysis showed that the difference of basic medium(WPM)ratio(1/2 WPM,1/4 WPM,1/8 WPM)in the culture medium on proliferation rate,pollution rate,rooting rate,seedling rate and root length reached a extremely significant level(P＜0.01,the same below),CO2 concentration on proliferation rate,rooting rate and root length difference reached an extremely significant level,and the effect of hormone ratio on the proliferation rate and rooting rate of blueberry open culture also reached an extremely significant level.The effects of matrix culture concentration and proportion on contamination rate and rooting rate reached a significant level(P＜0.05).Combined with the consideration of reducing pollution,the best proliferation culture formula for sugar-free culture of blueberries was 1/4 WPM,CO2 concentration of 1 000-1 500 μmom/mol,culture medium content of 80％in perlite matrix,hormone ratio of ZT:IBA was(0.5-1):2.(3)The test of the addition method of culture medium showed that the addition method of low and then high culture medium could reduce the contamination rate and significantly improve the proliferation rate.
BlueberriesSugar-free cultureCulture conditionsOpen culture systemOrthogonal test
万方数据
维普
