Effects of different carbon and nitrogen sources on liquid spawn growth of Lentinula edodes
Taking Lentinula edodes'Huxiang F2'as the experimental material,the effects of 9 carbon source formulas and 10 nitrogen source formulas on the growth rate of mycelium on solid medium,mycelium biomass and fermentation solid volume ratio in liquid medium,and the growth rate of mycelium in sawdust test tubes after fermentation were investigated.The results showed that on solid medium,when using molasses as the carbon source,the mycelial growth rate was the fastest,reaching 6.10 mm/d,while when using xylose as the carbon source,the mycelial growth rate was the slowest,only 3.84 mm/d;When using bran as the nitrogen source,the mycelial growth rate was 4.01 mm/d,while when using urea as the nitrogen source,the mycelial growth rate was the slowest,only 3.19 mm/d.In liquid medium,with molasses as the main carbon source,the mycelial biomass reached 8.04 g/L,and the fermentation solid volume ratio reached 98.69％;When using bran as the main nitrogen source,the mycelial biomass reached 7.90 g/L,and the fermentation solid volume ratio reached 96.57％.Correlation analysis showed that there was a significant positive correlation between mycelial growth rate and biomass on solid media with different carbon sources.In liquid media with different nitrogen sources,there was a significant positive correlation between mycelial biomass and the fermentation solid volume ratio.The mycelium biomass of different carbon and nitrogen sources was significantly positively correlated with the growth rate of mycelium inoculated in sawdust test tubes.In summary,molasses and bran were the optimal combination of carbon and nitrogen sources for L.edodes liquid spawn.The results could provide reference for the selection of raw materials for industrialized L.edodes liquid spawn.
Lentinula edodesLiquid spawnMycelial growth rateMycelial biomassVolume ratio of fermentation solids
万方数据
维普
