The present study had apical buds from the rhizome of American lotus(Nelumbo lutea)as explants,mainly focusing on exploring effective disinfection method and proper hormone and its concentration for the primary culture and also comparing the effects of different material collection time and temperature on in vitro culture for buds to grow into plantlets.It showed after the bud was disinfected,and removed off one leaf sheath,then disinfected again,the rest part or the rest part with another leaf sheath removed was cultured on medium containing 0.1％plant preservative mixture(PPM)or 250 mg/L cefotaxime sodium,the contamination rate could be reduced to around 10.71％-15.99％.Cultured on MS(Murashige and Skoog)basal medium containing 3 mg/L KT(Kinetin)and 0.2 mg/L NAA(1-Naphthaleneacetic acid)for one month and then transferred to medium containing 1 mg/L KT and 0.1 mg/L NAA or continuously cultured on 2 mg/L KT and 0.2 mg/L NAA,the bud explants could form plantlets and propagate effectively.The buds in dormant period during October and December were more easier to form plantlets than those in budding to pre-vegetative growth period during March and May.Incubation at 30 ℃ was more conducive for plantlets formation from buds than at 25 ℃.The in vitro culture system established in the study can provide genetically consistent plant materials and technical support for the popularization and application of this species and related experiments on American lotus as well.
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