Isolation of organic phosphate-solubilizing bacteria from rhizosphere soils of nine peony species and assessment of their phosphate-solubilizing capacity
Tree peony,an important native plant resource in China,has significant theoretical research,development and application values.Exploring resource-efficient and environment-friendly fertilization management pattern is more conducive to promote the green and sustainable development of the peony industry.In this study,the rhizosphere soil of 9 kinds of peony was used as the material,and the rhizosphere bacteria with the ability to solubilize organic phosphorus were isolated and screened by plate dilution method.The 16S rRNA gene sequencing technology was employed to identify these strains,and the phosphate solubilizing capability of these strains was evaluated by the combination of phosphate solubilizing circle method and the molybdenum antimony colorimetric method.The results showed that 14 strains of organic phosphate-solubilizing bacteria were screened from the rhizosphere soil of 9 kinds of peony,including 3 strains of Pseudomonas,3 strains of Arthrobacter,3 strains of Cupriavidus,2 strains of Bacillus,1strain of Rhodococcus,1 strain of Streptomyces and 1 strain of Azotobacter.The PSI(Phosphate solubilization index)and PSE(Phosphate solubilization efficiency)of different strains was 1.5-2.9 and 54.6％-194.6％,respectively.Moreover,the AP(Available phosphorus)content in the corresponding culture medium was 0.060-5.853 mg/L,and the mobilization rate of phytin was 30.48％-91.22％.In particularly,the strain PO15(belonging to Bacillus)was isolated from the rhizosphere soil of Yangshan peony with the highest PSI,PSE and AP content,suggesting that it has the strongest ability to degrade organic phosphorus.This study has important reference value for the screening of efficient phosphate-solubilizing strains and the development of special microbial fertilizer for peony,and provides new ideas and theoretical support for the green development of peony industry.
Tree peonyRhizosphereOrganic phosphatePhosphate-solubilizing bacteria
万方数据
维普
