摘要
比较了不同链格孢菌(Alternaria ochroleuca、Alternaria alternata 和 Alternaria tenuissima)的产毒能力,并通过单因素实验和响应面试验考察了不同的培养温度、培养时间和培养基初始pH对A.ochroleuca产主要链格孢霉毒素链格孢酚(AOH)、链格孢甲基醚(AME)和细交链格孢菌酮酸(TeA)3种毒素的影响.结果表明:3种毒素的产量均随培养温度、培养时间和培养基初始pH的增加呈先增加后逐渐减少的趋势,培养温度26.8 ℃、培养时间11.4 d、培养基初始pH 4.7是TeA的最佳产生条件,最大产量为4 841.3 mg/kg;当培养温度为25.9 ℃、培养时间为7.8 d、培养基初始pH为6.4时,AOH和AME的产量最高,分别达2 096.1 mg/kg和152.8 mg/kg.培养温度和培养基初始pH对TeA的产生具有显著的交互作用,而培养温度和培养时间对AOH和AME的产生具有显著的交互作用.本研究明确了链格孢菌A.ochroleuca产生链格孢霉毒素的主要影响因素,对控制链格孢霉毒素污染、保障食品安全具有重要意义.
Abstract
The toxin production ability of different Alternaria(Alternaria ochroleuca,Alternaria alternata and Alternaria tenuissima)was compared.The effects of culture temperature,culture time,and initial pH of the culture medium on the production of 3 major toxins,namely,alternariol(AOH),alternariol monomethyl ether(AME)and tenuazonic acid(TeA)of A.ochroleuca,were investigated through single factor experiments and response surface methodology.The results showed that the production of 3 toxins showed a trend of first increasing and then gradually decreasing with the increase of culture temperature,culture time,and initial pH of the culture medium.The optimal production conditions for TeA were culture temperature 26.8 ℃,culture time 11.4 days,and initial pH of the culture medium 4.7,with a maximum production of 4 841.3 mg/kg.When the culture temperature was 25.9 ℃,the culture time was 7.8 days,and the initial pH of the culture medium was 6.4,the production of AOH and AME was the highest,reaching 2 096.1 mg/kg and 152.8 mg/kg,respectively.The culture temperature and initial pH of the culture medium had a significant interaction effect on the production of TeA,while the culture temperature and culture time had a significant interaction effect on the production of AOH and AME.This study identified the main influencing factors of A.ochroleuca producing Alternaria toxin,which was of great significance for controlling Alternaria toxin contamination and ensuring food safety.
基金项目
上海市科技兴农重点攻关项目(2019-02-08-00-12-F01148)
维普
万方数据