小棚养殖凡纳滨对虾"滴星病"的研究
Study on"Dixing disease"of Litopenaeus vannamei cultured in small shed
于志君 1王李宝 2黎慧 2史文军 2赵然 1万夕和2
作者信息
- 1. 上海海洋大学水产科学国家级实验教学示范中心,上海 201306;江苏省海洋水产研究所,南通 226007
- 2. 江苏省海洋水产研究所,南通 226007
- 折叠
摘要
为探究凡纳滨对虾(Litopenaeus vannamei)"滴星病"的发病原因,从濒死的患病对虾肝胰腺中分离纯化得到一株优势菌MRY0520,并对菌株MRY0520进行了形态学观察和生理生化鉴定及分子生物学研究.结果表明:该菌株为美人鱼发光杆菌美人鱼亚种(Photobacterium damselae subsp.damselae),人工回感健康的凡纳滨对虾可致其出现与自然发病虾相同的症状.该菌对凡纳滨对虾72 h的半数致死量LD50为2.15 × 105 CFU/g,药敏试验结果显示MRY0520对新霉素(30 μg/片)、多西环素(30 μg/片)等多种药物敏感.该菌对凡纳滨对虾具有致病性,可致对虾肝胰腺出现明显的病理变化,甚至死亡.
Abstract
To explore the pathogenesis of Litopenaeus vannamei,a dominant strain MRY0520 was isolated from the hepatopancreas of dying diseased shrimp.Meanwhile,the strain MRY0520 was observed morphologically,identified physiologically and biochemically,and analyzed by molecular biology.The results showed that the strain MRY0520 was identified as Photobacterium damselae subsp.damselae,and the artificially infected L.vannamei could cause healthy shrimp exhibit the same symptoms as natural diseased shrimp.The results of artificial challenge showed that the LD50 of the bacteria to L.vannamei in 72 h was 2.15 x 105 CFU/g,the drug sensitivity test showed that strain MRY0520 was sensitive to neomycin(30 μg/disc),doxycycline(30 μg/disc)and other drugs.The bacteria was pathogenic to L.vannamei,and could cause obvious pathological changes in the hepatopancreas of the shrimp,even leading to death.
关键词
凡纳滨对虾/美人鱼发光杆菌美人鱼亚种/分离鉴定/药敏试验Key words
Litopenaeus vannamei/Photobacterium damselae subsp.damselae/Isolation and identification/Drug sensitivity test引用本文复制引用
基金项目
江苏省农业科技自主创新项目(CX213160)
江苏省现代农业产业技术体系建设项目(JATS2022419)
江苏省现代农业产业技术体系建设项目(JATS2022164)
江苏省农业重大新品种创制项目(PZCZ201747)
出版年
2024
国家科技期刊平台
NSTL
万方数据