转基因抗除草剂大豆ZH10-6转化体特异性定量检测体系的建立
Establishing an specific quantitative detection system for transgenic herbicide-resistant soybean ZH10-6 transformant
白蓝 1贾军伟 1陈一帆 1章寅 1潘爱虎 2吴潇3
作者信息
- 1. 上海市农业科学院生物技术研究所,上海 201106;上海市农业遗传育种重点实验室,农业农村部农作物生态环境安全检验测试中心(上海),上海 201106;上海市农业生物安全评价与检测专业技术服务平台,上海 201106
- 2. 上海市农业遗传育种重点实验室,农业农村部农作物生态环境安全检验测试中心(上海),上海 201106;上海市农业科学院畜牧兽医研究所,上海 201106
- 3. 上海市农业科学院生物技术研究所,上海 201106;上海市农业遗传育种重点实验室,农业农村部农作物生态环境安全检验测试中心(上海),上海 201106;上海市农业生物安全评价与检测专业技术服务平台,上海 201106;农业农村部农业转基因生物安全评价(环境)重点实验室,上海 201106
- 折叠
摘要
以新型转基因抗除草剂大豆ZH10-6外源插入片段的旁侧序列为靶标,建立了转化体特异性实时荧光PCR和单通道微滴式数字PCR精准定量检测体系,并邀请8家农业农村部检测中心对检测方法的准确性进行验证.结果表明:该转化体特异性实时荧光定量检测方法具有良好的特异性、重复性及广泛的适用性,定量极限达到50 copies/μL,检测极限达到5 copies/µL.该检测方法有望作为检测标准用于转基因抗除草剂大豆ZH10-6的定量检测,为该大豆品系的商业化应用及转基因标识等监管提供技术支撑.
Abstract
A precise quantitative detection system for transformant specific real-time fluorescence PCR and single channel micro drop digital PCR was established using the flanking sequence of the exogenous insertion fragment of the novel genetically modified herbicide-resistant soybean ZH10-6 as the target.Eight testing centers of the Ministry of Agriculture and Rural Affairs were invited to verify the accuracy of the detection method.The results showed that the transformant specific real-time fluorescence quantitative detection method had good specificity,repeatability,and wide applicability,with a quantitative limit of 50 copies/μL and a detection limit of 5 copies/μL.This detection method was expected to serve as a detection standard for the quantitative detection of genetically modified herbicide-resistant soybean ZH10-6,providing technical support for the commercial application and regulation of genetically modified labeling of this soybean variety(line).
关键词
抗除草剂大豆ZH10-6/转化体特异性/定量检测/实时荧光PCR/微滴式数字PCRKey words
Herbicide-resistant soybean ZH10-6/Transformant specific/Quantitative detection/Real-time PCR/Droplet digital PCR引用本文复制引用
基金项目
上海市农业科学院卓越团队建设计划项目(沪农科卓2022016)
出版年
2024
国家科技期刊平台
NSTL
万方数据