Effects of DNA shock on the viability of strawberry pollen
Taking'Shenqi'strawberry pollens as the materials,combined with different electrical pulse forms,electric intensity and electric shock times,the pollens were incubated in the dark at 28 ℃ on the pollen germination medium.The pollen germination was observed and analyzed under a microscope at 3,6,18,and 24 h to explore the appropriate parameters for electric shock treatment of strawberry pollen.The results showed that an 80 μs square wave pulse with 4.5-6.0 kV/cm electric intensity once reversibly inhibited pollen germination,and the inhibition degree was positively correlated with the electric intensity.The pollen germination rate after 3 h of electric shock was 36％—70％of CK1,and after 24 h it was 60％—85％of CK1.Partial reversible inhibition was generated by two times of electric shocks with a square wave intensity of 4.5-5.0 kV/cm,and the pollen germination rate was 20％—50％of CK1 after 24 h of electric shock.A square wave pulse of 5.5-6.0 kV/cm electric shock twice or 4.5-6.0 kV/cm electric shock 3 times resulted in irreversible inhibition,and the pollen germination rate was lower than 10％of CK1 after 24 h of treatment.After electric shocks with 2.75-6.0 kV/cm exponential decay wave for 3.5 ms,the pollen vitality rapidly decreased,and the pollen germination rate was below 1％after 18 h of electric shocks.In summary,a square wave pulse with an intensity of 4.5-5.0 kV/cm and an electric shock of 80 μs twice was the appropriate electric shock parameter for strawberry pollen.
Fragaria × ananassaDNA shockElectric intensityNumber of electric shocksPollen germination
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