Taking'Shenqi'strawberry pollens as the materials,combined with different electrical pulse forms,electric intensity and electric shock times,the pollens were incubated in the dark at 28 ℃ on the pollen germination medium.The pollen germination was observed and analyzed under a microscope at 3,6,18,and 24 h to explore the appropriate parameters for electric shock treatment of strawberry pollen.The results showed that an 80 μs square wave pulse with 4.5-6.0 kV/cm electric intensity once reversibly inhibited pollen germination,and the inhibition degree was positively correlated with the electric intensity.The pollen germination rate after 3 h of electric shock was 36％—70％of CK1,and after 24 h it was 60％—85％of CK1.Partial reversible inhibition was generated by two times of electric shocks with a square wave intensity of 4.5-5.0 kV/cm,and the pollen germination rate was 20％—50％of CK1 after 24 h of electric shock.A square wave pulse of 5.5-6.0 kV/cm electric shock twice or 4.5-6.0 kV/cm electric shock 3 times resulted in irreversible inhibition,and the pollen germination rate was lower than 10％of CK1 after 24 h of treatment.After electric shocks with 2.75-6.0 kV/cm exponential decay wave for 3.5 ms,the pollen vitality rapidly decreased,and the pollen germination rate was below 1％after 18 h of electric shocks.In summary,a square wave pulse with an intensity of 4.5-5.0 kV/cm and an electric shock of 80 μs twice was the appropriate electric shock parameter for strawberry pollen.
Fragaria × ananassaDNA shockElectric intensityNumber of electric shocksPollen germination
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