DNA电击对草莓花粉活力的影响
Effects of DNA shock on the viability of strawberry pollen
陈思宇 1杨秀蓉 2段可 1倪迪安 3李斌 4高清华5
作者信息
- 1. 上海海洋大学食品学院,上海 201306;上海市农业科学院林木果树研究所,上海市设施园艺技术重点实验室,上海 201403
- 2. 上海海洋大学食品学院,上海 201306;长江大学园艺园林学院,荆州 434000
- 3. 上海应用技术大学生态技术与工程学院,上海 201418
- 4. 上海市金山区农业技术推广中心,上海 201500
- 5. 上海市农业科学院林木果树研究所,上海市设施园艺技术重点实验室,上海 201403
- 折叠
摘要
以'申琪'草莓花粉为材料,结合不同电脉冲形态、电场强度和电击次数进行处理,然后在花粉萌发培养基上28 ℃黑暗孵育,于3、6、18、24h时用显微镜观察花粉萌发情况,探索草莓花粉电击适宜参数.结果表明:80 µs方波脉冲在4.5-6.0 kV/cm强度电击1次,可逆抑制花粉萌发,抑制程度与电场强度呈正相关;电击后3 h花粉萌发率为CK1的36％—70％,24 h时为CK1的60％—85％.方波4.5-5.0 kV/cm强度电击2次产生部分可逆抑制,电击24 h后花粉萌发率为CK1的20％—50％;5.5-6.0 kV/cm方波脉冲电击2次和4.5-6.0 kV/cm电击3次产生不可逆抑制,电击24 h后花粉萌发率低于CK1的10％.用2.75-6.0 kV/cm指数衰减波3.5 ms脉冲电击后,花粉活力快速下降,电击18 h后花粉萌发率在1％以下.综上,强度4.5-5.0 kV/cm方波脉冲80 μs电击2次为草莓花粉适宜电击参数.
Abstract
Taking'Shenqi'strawberry pollens as the materials,combined with different electrical pulse forms,electric intensity and electric shock times,the pollens were incubated in the dark at 28 ℃ on the pollen germination medium.The pollen germination was observed and analyzed under a microscope at 3,6,18,and 24 h to explore the appropriate parameters for electric shock treatment of strawberry pollen.The results showed that an 80 μs square wave pulse with 4.5-6.0 kV/cm electric intensity once reversibly inhibited pollen germination,and the inhibition degree was positively correlated with the electric intensity.The pollen germination rate after 3 h of electric shock was 36％—70％of CK1,and after 24 h it was 60％—85％of CK1.Partial reversible inhibition was generated by two times of electric shocks with a square wave intensity of 4.5-5.0 kV/cm,and the pollen germination rate was 20％—50％of CK1 after 24 h of electric shock.A square wave pulse of 5.5-6.0 kV/cm electric shock twice or 4.5-6.0 kV/cm electric shock 3 times resulted in irreversible inhibition,and the pollen germination rate was lower than 10％of CK1 after 24 h of treatment.After electric shocks with 2.75-6.0 kV/cm exponential decay wave for 3.5 ms,the pollen vitality rapidly decreased,and the pollen germination rate was below 1％after 18 h of electric shocks.In summary,a square wave pulse with an intensity of 4.5-5.0 kV/cm and an electric shock of 80 μs twice was the appropriate electric shock parameter for strawberry pollen.
关键词
草莓花粉/DNA电击/电场强度/电击次数/花粉萌发Key words
Fragaria × ananassa/DNA shock/Electric intensity/Number of electric shocks/Pollen germination引用本文复制引用
基金项目
上海市科技兴农项目(G2016060104)
上海市瓜果产业技术体系建设项目(沪农科产字2021第1号)
出版年
2024
国家科技期刊平台
NSTL
万方数据