超高效液相色谱-串联质谱法同时测定玉米和小麦中玉米赤霉烯酮和玉米赤霉烯酮-14-葡萄糖苷检测方法的建立
Development of a UHPLC-MS/MS method for simultaneous determination of zearalenone and zearalenone-14-glucoside in maize and wheat
蔡亚玲 1张志岐 2董芳 2聂冬霞 2韩铮 2李志忠 3赵志辉2
作者信息
- 1. 兰州理工大学生命科学与工程学院,兰州 730050;上海市农业科学院农产品质量标准与检测技术研究所,上海 201403
- 2. 上海市农业科学院农产品质量标准与检测技术研究所,上海 201403
- 3. 兰州理工大学生命科学与工程学院,兰州 730050
- 折叠
摘要
为科学防控和保障粮食质量安全,以玉米和小麦为材料,用乙腈/水/甲酸溶液提取,经HLB固相萃取柱净化,采用基质匹配标准溶液进行准确定量,建立了一种可靠的超高效液相色谱-串联质谱(UHPLC-MS/MS)方法,用于同时测定玉米和小麦中玉米赤霉烯酮(ZEN)和玉米赤霉烯酮-14-葡萄糖苷(ZEN-14G).结果表明:ZEN和ZEN-14G在0.1-200μg/L范围内呈良好线性关系(R2＞0.999),检出限为0.03-0.3 μg/kg,定量限为0.1-1.0 µg/kg.在 20、50 μg/kg 和 100 µg/kg 三种添加水平下,回收率为(80.2±9.8)％—(98.6±4.2)％,精密度为0.2％—9.1％(n=5).该方法准确、灵敏度高,可以满足玉米和小麦中ZEN和ZEN-14G的检测要求.
Abstract
To scientifically control and ensure food quality and safety,maize and wheat were used as materials,extracted with acetonitrile/water/formic acid solution,purified by HLB solid-phase extraction column,and accurately quantified by matrix matching standard solution,and a reliable ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS/MS)method was established for simultaneous determination of zearalenone(ZEN)and zearalenone-14-glucoside(ZEN-14G)in maize and wheat.The results showed that ZEN and ZEN-14G exhibited a good linear relationship in the range of 0.1-200 μg/L(R2＞0.999),with a detection limit of 0.03-0.3 µg/kg and a quantification limit of 0.1-1.0 pg/kg.At three levels of addition of 20,50 μg/kg,and 100 µg/kg,the recovery rate was(80.2±9.8)％—(98.6±4.2)％,and the precision was 0.2％—9.1％(n=5).This method is accurate,highly sensitive,and can meet the detection requirements of ZEN and ZEN-14G in maize and wheat.
关键词
玉米赤霉烯酮/玉米赤霉烯酮-14-葡萄糖苷/超高效液相色谱-串联质谱法/玉米/小麦Key words
Zearalenone/Zearalenone-14-glucoside/UHPLC-MS/MS/Maize/Wheat引用本文复制引用
基金项目
上海市科技兴农技术创新项目(2020-02-08-00-12-F01453)
上海市农科院卓越团队项目(沪农科卓20220011)
出版年
2024
国家科技期刊平台
NSTL
万方数据