Cloning and expression analysis of IbTOE3-like gene in sweet potato
Using the sweet potato variety'Xiguahong'as the material,the full-length CDS sequence of the IbTOE3-like gene was obtained through homologous cloning,and the physicochemical properties and basic functions of its encoded protein were analyzed.The results showed that the transcription level of IbTOE3-like gene was higher in sweet potato root tuber compared to other tissues,and increased with the development of sweet potato root tuber confirming it as a sweet potato root tuber swelling related gene.The IbTOE3-like protein had two connected AP2 domains and belonged to the AP2/ERF family.It was predicted to be a hydrophilic protein.The tobacco transient transformation test determined that the IbTOE3-like protein was located in the nucleus.Transcriptional activation activity validation revealed that the IbTOE-like gene had no transcriptional activation activity.In addition,transient overexpression of miR172 in tobacco leaves could effectively inhibit the transcription level of IbTOE3-like gene,and the targeted relationship between miR172 and IbTOE3-like gene was demonstrated through the 5'RACE assay.The results could lay the foundation for further research on the role of IbTOE3-like gene in the process of sweet potato root tuber swelling.
万方数据
维普
