Nutrient abundance and protein quality of fifteen market-available Pleurotus pulmonarius from China and Malaysia
Fifteen market-available Pleurotus pulmonarius samples were collected from China or Malaysia.The nutrient profile and protein quality were analyzed and compared.Principal component analysis(PC A)was used to extract comprehensive components from data pool,which elucidated sample variance through a dimensionality-reduction approach.The nutritional quality and amino acid balance of P.pulmonarius proteins were systematically evaluated based on the current international reference amino acid pattern.The results showed that,as measured by dry weight,P.pulmonarius contained 20.29-42.86 g/(100 g)of protein,2.29-7.07 mg/g of crude polysaccharide,8.47-14.76 mg/g of crude fiber.The amino acids,crude fiber,and fat were of higher content in P.pulmonarius from China,while Malaysian products were more abundant in polysaccharide and selenium.The 27 nutrients were standardized by PCA,and 5 principal components(PC)were extracted,which totally explained 91.45%of sample variance.Scoring analysis suggested the samples from China and Malaysia scored higher in PC1 and PC3 respectively.Comprehensively,samples from China were more uniform than samples from Malaysia with larger nutritional divergence.The total IAA of P.pulmonarius protein met the requirements of reference amino acid patterns by WHO and IOM.Sulfur-containing amino acids were the dominant amino acids with the highest amino acid score,IOM score,chemical score and ratio coefficient.Valine and leucine obtained the minimum scores.His and Phe+Tyr scored lower in Malaysian samples as compared with Chinese samples.The digestibility corrected amino acid score(PDCAAS)of all tested samples ranged from 79.31 to 104.70,and the essential amino acid index(EAAI)ranged from 80.38 to 96.14 indicating differences in protein quality across producers.The findings of this work provide references for the deep-processing of P.pulmonarius as well as establishing a standardized quality assessment system.