摘要
对16种常见的食用菌醇提物体外抗氧化和抗炎生物活性进行初步比较研究.结果表明:瓦尼桑黄醇提物具有良好的体外1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picryhydrazyl,DPPH)和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[(2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulphonate),ABTS]自由基清除能力,在0.05 mg/mL浓度下,其对DPPH和ABTS清除率分别可达到87.21％和93.46％.相关性分析显示,抗氧化活性与总黄酮和总酚含量呈正相关.此外,蟹味菇、杏鲍菇、瓦尼桑黄、灵芝、香菇、白玉菇、茯苓、猪苓、猴头菌、羊肚菌、松茸菌、蛹虫草、鹿茸菇、裂褶菌、樟芝菌醇提物均可不同程度地抑制脂多糖诱导巨噬细胞RAW 264.7 一氧化氮和活性氧的分泌,阻滞该细胞的吞噬能力,表现出较好的抗炎活性.综上,本研究为筛选具有抗氧化和抗炎活性的食用菌资源提供了理论依据,为相关产品的研发提供了科学参考.
Abstract
The antioxidant and anti-inflammatory bioactivities of alcohol extracts from sixteen common edible fungi were studied.The results showed that the alcohol extract of Sanghuangporous vaninii possessed excellent in vitro free radical scavenging ability for 1,1-diphenyl-2-picrylhydrazine(DPPH)and 2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulphonate)(ABTS),and its scavenging rates for DPPH and ABTS could reach 87.21％and 93.46％,respectively,at a concentration of 0.05 mg/mL.Correlation analysis showed that antioxidant activity was positively correlated with total flavonoid and total phenolic contents.Additionally,the alcohol extracts of Hypsizygus marmoreus,Pleurotus eryngii,Sanghuangporous vaninii,Grifola frondosa,Ganoderma lucidum,Lentinus edodes,White hypsizygus marmoreus,Poria cocos,Polyporus umbellatus,Hericium erinaceus,Morchella esculenta,Tricholoma matsutake,Cordyceps militaris,Lyophyllum decastes,Schizophyllum commune and Antrodia cinnamomea could inhibit the secretion of nitric oxide and reactive oxygen species from macrophage cells RAW 264.7 induced by lipopolysaccharides to varying degrees,and blocked the phagocytic abilities of these cells,thus showing good anti-inflammatory activity.In summary,this study provides a theoretical basis for screening edible fungi resources with antioxidant and anti-inflammatory activities,and provides a scientific reference for the development of related products.
维普
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