Comparison of the effects of ultraviolet irradiation on two porcine epidemic diarrhea virus strains infecting cells in vitro
Porcine epidemic diarrhea virus(PEDV)is one of the important causes of diarrhea and death in piglets,and up to date,there are no effective therapeutic drugs and vaccines for PEDV.Different strains of PEDV differ in the way infecting cells and in the rate of viral proliferation.Currently,preventive management measures such as disinfection and sterilization are mainly relied upon to prevent virus transmission and contamination.In this study,we applied the trypsin-free method to infect the adherent cultured cells with CV777 and DR13 strains of PEDV in vitro separately,and tested the inactive effect of ultraviolet irradiation(UV)on different strains.The results showed that 100 TCID50 DR13 and CV777 strains were inactivated using a UV energy value of 48.6 mJ/cm2 and 10 000 TCID50DR13 and CV777 strains were inactivated using a UV energy value of 291.6 mJ/cm2.This study is of great significance for the prevention and control of PEDV.
万方数据
维普
