Relationship between the production of prototypic and modified deoxynivalenol and TRI gene expression under different environmental factors
Wheat was inoculated with Fusarium graminearum to study the production of prototypic and modified deoxynivalenol(DON)and the expression of key TRI genes,such as TRI3,TRI5,TRI6,TRI8,etc.,at different temperature(10,20,30 ℃),water activities(0.95,0.98 aw),and incubation time(7,14,21,28,35 d).Then the relationship between the two,as well as the effects of single and interactive environmental factors were analyzed.The results showed that the optimal temperature and water activity for the production of prototypic and modified DON were both 20 ℃ and 0.98 aw,and most genes were expressed the highest under these conditions.The expression of TRI5 gene was significantly correlated with the production of DON and 15-acetyl-deoxynivalenol(15-ADON),and the expression of TRI6 and TRI8 genes was significantly correlated with the production of DON,3-acetyl-deoxynivalenol(3-ADON)and 15-ADON,whereas TRI3 expression was not significantly correlated with the production of the 4 toxins,and deoxynivalenol-3-glucoside(D3G)production was not significantly correlated with the expression of TRI genes.The single factors of temperature,water activity,and cultivation time,as well as their interactions,could significantly affect the production of DON and the expression of TRI genes,while modified DON was more significantly affected by most environmental factors.This study could provide theoretical and scientific basis for the regulation,prevention and control of prototypic and modified DON mycotoxins in agricultural products.
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维普
