Isolation,identification and characteristics analysis of high-yield amylase producing strains from pit mud and distiller's grains of Luzhou-flavor Baijiu
The unique flavor and taste of Luzhou flavor Baijiu are inseparable from the role of functional microorganisms in the brewing environment.This study used transparent circle method and amylase activity determination to isolate high-yield amylase strains from the pit mud and distillers grains of Luzhou flavor Baijiu.Through microscope and colony morphology observation,physiological and biochemical analysis,PCR amplification of 16S rDNA gene and NCBI blast,a phylogenetic tree was preliminarily constructed.Then,characteristic analysis was conducted on four strains.The results showed that the four strains belonged to the genus Bacillus,and the highest amylase activity reached 235.78 U/mL.The homologous strains are Bacillus cereus,Bacillus amyloliquefaciens,Nialia circulans and Bacillus licheniformis.Through in-depth research on other properties,it was found that they all have the ability to decompose cellulose and have different antibacterial effects on Escherichia coli,Salmonella,and Staphylococcus aureus.
万方数据
维普
