Objective To explore the molecular mechanism of PM2.5 induced lung injury in order to provide a new target for the diagnosis and treatment of lung injury.Methods Differential expression analysis and molecular function analysis were performed on the multi-omics data to identify the molecular targets related to PM2.5-induced lung injury.PM2.5-induced lung injury model was established in SD rats,and the molecular targets related to lung injury was validated.Results HE staining showed that the alveolar interval was widened,the alveolar cavity was enlarged,and part of the alveolar was broken and fused in the rats exposed to PM2.5.Masson staining showed a small amount of fibrosis in the lungs of exposed rats.Results showed that over-expression of miR-122 significantly down-regulated the expression of its target gene IGF1R under PM2.5 induction.Conclusion The study indicates that miR-122 may affect the PI3K-AKT signaling pathway and induce apoptosis by regulating IGF1R to aggravate lung injury in rats exposed to PM2.5.
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