Objective To investigate the therapeutic effect and related mechanisms of Fu's subcutaneous needling(FSN)for skeletal muscle contusion in mice.Methods C57/BL6 male mice were randomly divided into 4 groups:control group,model group,FSN group and acupuncture(ACP)group.Except for control group,the left gastrocnemius muscle of mice was injured by contusion.The FSN group received FSN treatment plus reperfusion once every alternative day for 6 times;the ACP group received acupuncture at the acupoint Zusanli(ST 36).The changes in hyperalgesia were evaluated by von Fery test and Hargreaves test;the degree of muscle edema was assessed by measuring the wet weight and circumference of gastrocnemius muscles;inflammation and fibrosis were evaluated by histology and RT-qPCR;and subpopulations of macrophage were evaluated using flow cytometry.Results The thermal withdrawal latency significantly prolonged in FSN and ACP group.FSN treatment significantly reduced the degree of muscle edema,and acupuncture treatment has no effect.FSN treatment reduced the gene expression of inflammatory factors and infiltrating leukocytes on the 3 rd day.Meanwhile,it decreased the fibrotic area of damaged muscles on 7th day and 14th day.The number of Ml macrophages decreased,while the number of M2 macrophages increased after FSN treatment on 7th day.The macrophage subgroup in the acupuncture did not change significantly.Conclusion The study suggests that FSN treatment not only alleviate thermal hyperalgesia and reduce early inflammation levels and muscle edema,but also reduce fibrosis in mice with skeletal muscle contusion,which may be achieved by promoting M2 macrophage polarization.
万方数据
维普
