A study on central neural pathway of defecation regulation in rats
Objective To investigate higher central neural pathways of defecation reflex with pseudo rabies virus(PRV)tracing and calcium imaging techniques.Methods Adult male SD rats were divided into an experimental group and a control group.The rats in experimental group were injected with PRV virus encoding fluorescent protein into the rectal wall,while the control group rats were injected with empty vector virus.After 5 days of injection,the rats were euthanized and rectal and brain tissues were harvested for fluorescence quantitative analysis.At the same time,using gene encoded calcium ion indicators(GCaMP)and in vivo calcium imaging technology,the dynamic changes of calcium signals in defecation activity related neurons were observed.Results The hypothalamus of the rats in experimental group showed gradually increasing fluorescence signals.Through graphic analysis,it was confirmed that the vertebral neurons were upstream synaptic connecting neurons in the rectum.Through in vivo calcium imaging technique,it was further confirmed that the calcium signal of neurons in the hypothalamus area increased significantly during defecation activity.Conclusion This study preliminarily identified the localization of centers related to defecation reflex in rats,which provides an experimental basis for further study on the neural regulatory mechanisms of defecation reflex.
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