Objective:To investigate and analyze the expression of neural cell adhesion molecule(NCAM)and cellu-lar oncogene fos(c-fos)in CA1 region of hippocampus after ischemic brain injury under the influence of astragalan(AG).Methods:In this study,200 Wistar male rats were randomly divided into 4 groups.The rats in sham operation group(SOG,n=20)underwent sham operation without ischemic treatment.The rats in model group(MG,n=60)(MG-1 d;MG-3 d;MG-7 d)were given ischemic treatment to simulate brain injury and further subdivided into 3 time points of 1 day,3 days,and 7 days.The rats in low-dose astragalus polysaccharides treatment group(L-AGTG,n=60)(L-AGTG-1 d;L-AGTG-3 d;L-AGTG-7 d)were given low-dose astragalus polysaccharides treatment after ischemia and further di-vided into 3 time points of 1 day,3 days,and 7 days.The rats in high-dose astragalus polysaccharides treatment group(H-AGTG,n=60)(H-AGTG-1 d;H-AGTG-3 d;H-AGTG-7 d)were given high-dose astragalus polysaccharides treatment after ischemia and further divided into 3 time points of 1 day,3 days,and 7 days.In the MG and AGTG groups,the rat brain tissue was in state of ischemia induced by neck incision,and then the right middle cerebral artery was closed through neck resection.In AGTG group,AG was injected intraperitoneally at 5 mg/kg and 15 mg/kg,respectively.The ce-rebral blood flow was reperfused on the 1st,3rd and 7th day.After the degree of nerve injury was evaluated,the specimens were collected and the number of apoptotic neurons was counted.Immunohistochemistry and reverse transcription-polymerase chain reaction(RT-PCR)were used to detect the expression of NCAM and c-fos in hippocampal CA1 region neurons.Results:Compared with those in MG group,the neurological impairment index(NIPs)scores and the NCAM of apoptotic neurons in hippocampus of L-AGTG group and H-AGTG group were significantly decreased,and the differences were statistically significant(P<0.05,P<0.01).In the CA1 region of hippocampus,the expression levels of NCAM and c-fos protein in L-AGTG group and H-AGTG group were higher,and the differences were statistically significant(P<0.05,P<0.01).The expression profiles of beta-actin and c-fos mRNA in hippocampal CA1 region of L-AGTG group and H-AGTG group also showed significant changes,indicating that AG had a positive regulatory effect on the expression of c-fos mRNA.Conclusion:Studies have shown that AG can improve ischemic brain damage,which may be related to promoting the expression of hippocampal MSAM and c-fos,and preventing or reversing hippocampal neuron apoptosis.
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