Exploration of the mechanism of Xuming Decoction in the treatment of stroke in Gujin Luyan based on network pharmacology
Objective:To explore the potential mechanism of Xuming Decoction in the treatment of stroke in Guj in Luyan by network pharmacology.Methods:The active ingredients and their targets of Mahuang,Danggui,Guizhi,Renshen,Xingren,Shigao,Chuangxiong,Ganjiang and Gancao in Xuming Decoction in Guj in Luyan were searched through TCMSP database."Cerebral infarction"was used as the key word to search OMIM,GeneCards and NCBI database for effective human gene retrieval.Venny 2.1 software was used to produce Venn diagram of component targets and disease targets.The PPI net-work diagram was constructed through the STRING database and introduced into Cystoscape 3.8.0 software.The network ana-lyzer method was used to complete the topology analysis,and the degree ranking method was used to select the targets whose score exceeded the average as the main targets.The MCODE module was started for genomic data analysis and core target se-lection.Cytoscape 3.8.0 software was used to construct component-disease-target network diagram and perform topological analysis to rank the components by degree.GO functional enrichment analysis and KEEG pathway enrichment analysis were used for simple annotation of genes.R language and cluster profiler package were used to create column diagrams and bubble diagrams to obtain more accurate data results.Results:The active ingredients of Xuming Decoction in Guj in Luyan had 256 targets,1841 targets for stroke,and 158 targets for intersection between ingredients and diseases.PPI network structure com-bined with MCODE algorithm were used to screen out 10 gene clusters and 9 key genes in the treatment of stroke by Xuming Decoction,including the cholinergic receptor muscarine 1(CHRM1),caspase 9(CASP9),signal transducer and activator of transcription proteins(STAT),hypoxia-inducible factor(HIF),oxidized low-density lipoprotein receptor 1(OLR1),UDP glucuronyltransferase 1 family polypeptides A1(UGT1A1),plasminogen activator(t-PA),interferon regulatory factor 1(IRF1),and matrix metallopeptidase 3(MMP3),etc.GO functional enrichment analysis enriched 2458 biological pro-cesses,174 molecular functions and 109 cellular components.KEGG pathway enrichment analysis obtained 177 signaling pathways.Through the combination of active ingredients quercetin,luteolin,kaempferol and other key targets,the Xuming Decoction acted on blood lipids and aging signaling pathways in atherosclerosis and diabetes complications to achieve effective treatment of stroke.Conclusion:The Xuming Decoction in Guj in Luyan has diversified therapeutic characteristics.The re-search of network pharmacology points out the way for the basic theoretical research and practical application.
strokeXuming DecoctionGujin Luyannetwork pharmacologymechanism of action
NETL
NSTL
万方数据
维普
