Exploration of the mechanism of"Huangqi-Haifengteng"in the treatment of henoch-schonlein purpura nephritis by network pharmacology and molecular docking
Objective:To explore the mechanism of couplet medicines of"Huangqi-Haifengteng"in the treatment of henoch-schonlein purpura nephritis(HSPN)by network pharmacology and molecular docking based on the method of toni-fying Qi and dredging collaterals.Methods:The active ingredients and drug targets of Huangqi and Haifengteng were searched through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and then the disease targets of HSPN were searched through GeneCards and DisGeNET databases.Then the intersection targets of active ingredients and diseases were selected.Cytoscape software was used to construct the PPI network of active ingredients-intersection gene targets,and DAVID database was used for gene function GO and KEGG pathway enrichment analysis.Autodock software was used to perform molecular docking between key active ingredients and core targets.Re-sults:Ten active ingredients of Huangqi and 18 active ingredients of Haifengteng were screened.205 effect targets and 795 targets of HSPN disease were identified.There were 73 common target genes between drugs and HSPN.GO functional analy-sis involved the regulation of negative feedback of apoptosis process and tumor necrosis factor receptor binding,and KEGG pathway enrichment analysis involved tumor necrosis factor(TNF)and interleukin-17(IL-17)signaling pathways.Mo-lecular docking showed that the active ingredients quercetin,stigmasterol,and kadsurenone in the top four couplet drugs with the highest connectivity had good binding activity to the key core targets matrix metallopeptidase-9(MMP-9),TNF,and tumor protein P53(TP53).Conclusion:This study preliminarily verifies the mechanism of"Huangqi-Haifengteng"in the treatment of HSPN,and provides scientific basis for further research.
HSPNtonifying Qi and dredging collateralsHuangqi-Haifengtengnetwork pharmacologymolecular docking
NETL
NSTL
万方数据
