Effects of electropuncture at different acupoint groups on Gasdermin-D in hippocampus of middle cerebral artery occlusion model rats
Objective:To observe the effects of electropuncture at Bei Shu(Ganshu and Shenshu),Yangming Merid-ian(Quchi and Zusanli)and Du Mai(Shenting and Baihui)on nerve function,learning and memory ability and Gasdermind-D mRNA expression in hippocampus of middle cerebral artery occlusion(MCAO)model rats,and to explore its possible mechanism.Methods:Thirty SD rats were randomly divided into sham-operated group,model group,Bei Shu acupoint group,Yangming Meridian acupoint group and Du Mai acupoint group,with 6 rats in each group.The MCAO rat model was made by reforming Longa method.The rats in the Bei Shu acupoint group,Yangming Meridian acupoint group and Du Mai acupoint group were given electropuncture for 20 min every day for 7 consecutive days for on 24 h after model-ing.The rats in the model group and the sham-operated group were captured under the same conditions.The modified neu-rological severity score(mNSS)was used to evaluate the neurological impairment in rats.Morris water maze experiment was used to detect the learning and memory ability of rats.Real-time quantitative fluorescent PCR(q-PCR)was used to detect Gasdermin-D mRNA expression in hippocampus.Results:In terms of mNSS score,the mNSS score of rats in the model group and the three electropuncture groups were higher than those in the sham-operated group on the first day after model-ing,and the difference was statistically significant(P<0.05).There was no statistical significance in mNSS scores between the model group and the three electropuncture groups.On the 7th day after modeling,the scores of the Yangming Meridian acupoint group and Du Mai acupoint group were significantly lower than those of the model group and the Bei Shu acupoint group,and were lower than those of the same group on the 1st day after modeling,with statistical significance(P<0.05).In the Morris water maze directional navigation test,compared with the sham-operated group,the total time of platform explo-ration in the model group and the Bei Shu acupoint group increased on the 4th day,and the Yangming Meridian acupoint group increased on the 3rd and 5th day,with statistical significance(P<0.05).Compared with the model group,the Yang-ming Meridian acupoint group and the Du Mai acupoint group had a significant reduction in the total time of platform explo-ration on the 4th day,and the Bei Shu acupoint group had a significant reduction on the 3rd and 6th day,with statistical sig-nificance(P<0.05).Compared with the Bei Shu acupoint group,the Yangming Meridian acupoint group and the Du Mai acupoint group had a significant reduction in the total time of platform exploration on the 4th day,and the difference was statistically significant(P<0.05).Compared with Yangming Meridian acupoint group,the total exploration time of Du Mai acupoint group on the 4th day was lower,and the difference was statistically significant(P<0.05).In the space exploration experiment,compared with the sham-operated group,the number of times of crossing the platform in the model group and Bei Shu acupoint group decreased,and the difference was statistically significant(P<0.05).Compared with the model group,the number of times of crossing the platform increased in Yangming Meridian acupoint group and Du Mai acupoint group,and the difference was statistically significant(P<0.05).In q-PCR experiment,the relative expression level of Gasdermin-D mRNA in model group and Bei Shu acupoint group was higher than that in sham-operated group,and the dif-ference was statistically significant(P<0.05).The mRNA relative expression of Gasdermin-D in Yangming Meridian acu-point group and Du Mai acupoint group was lower than that in model group and Bei Shu acupoint group,and the difference was statistically significant(P<0.05).Conclusion:Electropuncture in Du Mai acupoint group and Yangming Meridian acu-point group can improve nerve function scores,alleviate symptoms of nerve function deficit,improve learning and memory ability,and reduce Gasdermin-D mRNA expression in hippocampus,and the effect is better than that in Bei Shu acupoint group.The mechanism may be related to the down-regulation of Gasdermin-D mRNA expression in hippocampus.
MCAOelectropunctureBei Shu acupointYangming MeridianDu MaiGasdermin-D
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