Identification of the blood-entry components of heterophyllin based on UPLC-Q-TOF-MS technique
Objective:To analyze the major blood-entry components of Heterophyllin.Methods:Male SPF-grade SD rats were orally administered Heterophyllin extract,and blood samples were collected from the orbital sinus at different time points.Plasma samples were separated by centrifugation and analyzed using ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS).A Waters ACQUITY UPLC® CSH C18(2.1×100 mm,1.7 μm)column was used,with acetonitrile and 0.1%aqueous formic acid as the mobile phases under gra-dient elution.Based on the molecular ions and fragment ion characteristic peaks,the prototype components in blood and me-tabolites of different cyclotides in the rat were analyzed.Results:After oral administration of Heterophyllin extract to rats,a total of 15 blood-entry components were detected by LC-MS analysis,including 7 prototype components and 8 metabo-lites.The metabolic pathways mainly included phase I hydrolysis,demethylation to carboxylic acid,dehydrogenation,hy-drogenation,and phase II methylation conjugation reactions.Conclusion:The blood-entry prototype components and me-tabolites of Heterophyllin were preliminarily identified,providing data support for further studies on the pharmacological material basis and quality control.
万方数据
维普
