Study on the growth inhibitory effect of oxymatrine on human ovarian cancer CAOV3 cells and its mechanism
Objective:To investigate the growth inhibitory effect of oxymatrine on human ovarian cancer CAOV3 cells and its underlying mechanism.Methods:Human ovarian serous epithelial cancer cell line CAOV3 cells were cultured in vi-tro.Oxymatrine was applied at concentrations of 0 mg/ml,2 mg/ml,4 mg/ml,and 8 mg/ml for 24 hours and 48 hours.The cell proliferation capacity was detected using the thiazolyl blue(MTT)method to determine the optimal concentration and duration of drug action.Scratch assays were conducted using 4 mg/ml oxymatrine to evaluate cell migration.Western blotting was used to measure the protein levels of Caspase3,Bax,and Bcl-2.Flow cytometry was employed to assess the cell apop-tosis rate.Results:Compared to the blank group,oxymatrine significantly reduced the viability of CAOV3 cells,with the inhibitory effect being time-and dose-dependent,with statistically significant differences(P<0.05).The optimal effective condition was determined to be 4 mg/ml oxymatrine for 48 hours.Compared to the blank group,oxymatrine inhibited the mi-gration of CAOV3 cells,with the effect being time-dependent,and the difference was statistically significant(P<0.05).Oxymatrine increased the expression of Caspase3 and Bax proteins and decreased the expression of Bcl-2 protein in CAOV3 cells,and the difference was statistically significant(P<0.05).Oxymatrine promoted early apoptosis in CAOV3 cells.Con-clusion:Oxymatrine may inhibit the growth and migration of CAOV3 cells by promoting early apoptosis,and its mechanism may involve promotion of Caspase3 and Bax protein expression and inhibition of Bcl-2 protein expression.
CAOV3 cellsoxymatrineinhibitory effectmechanism study
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维普
