Mechanism research of reduction of inflammatory injury in the paraventricular nucleus in spontaneously hypertensive rats with white matter lesions by the effect of acupuncture on regulating the JNK-MCP1 pathway
Objective:To investigate the therapeutic effect of acupuncture at"jiangyafang"acupoint on inflammatory injury of paraventricular nucleus(PVN)in spontaneously hypertensive rats(SHR)in the mode of two blood vessels gradual occlusion(2VGO),and to explore its related mechanism from the angle of c-Jun N-terminal kinase(JNK)and monocyte chemotactic protein-1(MCP-1)pathways.Methods:30 SHR rats were selected.,10 SHR rats were randomly selected to separate only bilateral common carotid arteries as sham-operated group;another 20 SHR rats were implanted with vasocon-strictor reducing rings to narrow the common carotid arteries,and SHR white matter lesions model with two blood vessels gradual occlusion(SHR-2VGO)was made.The rats with impaired motor function were excluded after 2 weeks of model se-lection.,18 SHR-2VGO rats after successful molding were randomly divided into model group(9 rats)and acupuncture group(9 rats).The rats in the sham-operated group and the model group were fixed in the same way as the rats in the acu-puncture group.At the same time,the rats in the acupuncture group were acupunctured at"jiangyafang"acupoint for once a day,30 min each time,and the intervention course was 4 weeks.The systolic and diastolic blood pressure of rats were mea-sured weekly by non-invasive blood pressure measurement system,and the antihypertensive effect of acupuncture was evaluated.The histopathological changes of PVN in each group were evaluated by HE staining and Nissl staining.The ex-pressions of JNK and MCP1 in PVN in each group were detected by immunofluorescence method.Real-time fluorescence quantitative PCR was used to detect the relative expression of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)mRNA in PVN tissue of rats in each group.Results:Compared with sham-operated group,systolic blood pressure and dia-stolic blood pressure of rats in model group were significantly increased before acupuncture and at the 1st,2nd,3rd and 4th week of observation period(P<0.05);at the 3rd and 4th week of the observation period,compared with the model group,the systolic blood pressure and diastolic blood pressure of rats in the acupuncture group were significantly decreased(P<0.05).The distribution of large and small neurosecretory cells in the encephalic region of PVN of the sham-operated group was regular.The two types of cells showed sparse distribution and irregular shape in the model group,while the two types of cells showed cross arrangement in the acupuncture group.The number of Nissl bodies in the encephalic region of PVN of rats in the sham-operated group was abundant and the volume was large;in the model group,the number of Nissl bodies in the PVN was significantly decreased,and the nerve cells were significantly damaged;the volume of Nissl bodies in the acu-puncture group was smaller,but the number was significantly increased compared with that of the model group.Compared with that of the sham-operated group,the fluorescence intensity of JNK-MCP1 in PVN was significantly increased in the model group(P<0.05);compared with that of the model group,the fluorescence intensity of JNK-MCP1 was decreased in the acupuncture group(P<0.05).Compared with those of the sham-operated group,the TNF-α and IL-6 mRNA levels in PVN tissues in the model group were significantly increased(P<0.05);compared with those of the model group,the relative expression levels of TNF-α and IL-6 mRNA in PVN tissues in the acupuncture group were significantly decreased(P<0.05).Conclusion:Acupuncture at"jiangyafang"acupoint can alleviate the pathological injury of PVN in SHR-2VGO rats,increase the number of Nissl bodies,and alleviate the inflammatory injury of PVN in SHR-2VGO rats by regulating JNK-MCP1 pathway.Acupuncture alleviates neuroinflammation associated with hypertensive white matter lesions,which is a potential treatment strategy worth studying.
spontaneous hypertensionwhite matter lesions"jiangyafang"acupointinflammatory injury in PVNJNK-MCP1 pathway
万方数据
维普
