Evaluation of synergistic effect of electroacupuncture combined with Omeprazole on gastric ulcer protection in NSAIDs rats
Objective:To evaluate the synergistic effect of electroacupuncture at Zusanli acupoint combined with dif-ferent doses of Omeprazole on the protection of Indomethacin-induced gastric ulcer in rats.Methods:48 male SD rats were randomly divided into 8 groups:blank group,model group,low-dose Omeprazole(5 mg/kg,Omeprazole L)group,medium-dose Omeprazole(10 mg/kg,Omeprazole M)group,high-dose Omeprazole(20 mg/kg,Omeprazole H)group,electroacupuncture combined with low-dose Omeprazole(E+Omeprazole L)group,electroacupuncture combined with medium-dose Omeprazole(E+Omeprazole M)group,and electroacupuncture combined with high-dose Omeprazole(E+Omeprazole H)group,with 6 rats in each group.At 1 h before the last administration,the gastric ulcer model was prepared by Indomethacin(40 mg/kg intraperitoneally injected)in all groups except the blank group.The gross appearance of gastric mucosa and pathological morphology of gastric and intestinal tissues were observed 7 h after modeling,and the appearance and pathological damage of gastric mucosa were scored.Results:In the model group,the gastric mucosa was seriously damaged,and the mucosal surface was partially thinner,with linear bleeding,erosion or foci of ulcer.The pathological changes were mainly red cell and epithelial cell degeneration defect in the mucosal layer.The pathological changes of small intestinal mucosal lesions were slightly damaged,and the villous striate margin was shed and the epithelial cells vacuolated.(1)Compared with the model group,the gross appearance and pathological changes of gastric mucosa in each intervention group were improved,and the gastric mucosal injury score was decreased(P<0.05,P<0.01).Except for Omeprazole L group,the pathological scores of other groups were lower than those of model group(Omeprazole H group P=0.08,the rest P<0.01).The damage inhibition rate was the highest in the Omeprazole M group,E+Omeprazole M group and E+Omeprazole H group.(2)Comparison among the three doses groups of Omeprazole:compared with Omeprazole L group and Omeprazole H group,the gross appearance and pathological changes of gastric mucosa were significantly reduced,and the gastric muco-sal injury score and pathological score were decreased in Omeprazole M group(P<0.01,P<0.05).(3)Comparison among the three doses groups of E+Omeprazole:compared with E+Omeprazole L group,the gross appearance injury of gastric mu-cosa was reduced,and the gastric mucosal injury score were significantly decreased in E+Omeprazole M group and E+Omeprazole H group(P<0.05,P<0.01).(4)Comparison among the same dose of Omeprazole groups and the E+Omeprazole groups:compared with the same dose of Omeprazole group,the gross appearance and pathological changes of gastric mu-cosa in E+Omeprazole L group and E+Omeprazole H group were alleviated,the damage scores were decreased(P<0.05,P<0.01),and the pathological scores showed a decreasing trend(P=0.1,P=0.08).(5)In the model group,the small intestinal mucosa was slightly damaged,the villous striate margin was partially disappeared and exfoliated,and the epithelial cyto-plasm vacuolated.Compared with the model group,the pathological changes of small intestinal mucosa in Omeprazole H group were significantly aggravated,including mucosal bleeding,degeneration and necrosis of epithelial cells and disinte-gration of lamina propria.There was no aggravation of intestinal mucosal injury in other groups.Conclusion:Electroacu-puncture combined with low-dose and high-dose Omeprazole has protective and synergistic effect on gastric mucosa of Indomethacin-induced acute gastric ulcer in rats.The combined application of electroacupuncture can prevent high-dose of Omeprazole from aggravating small intestinal injury caused by Indomethacin.
gastropathy of NSAIDscombination of acupuncture and medicineZusanli acupointOmeprazolesyner-gistic effect
万方数据
维普
