Optimization of extraction process of general flavone of Wuzhimaotao by response surface method and comparative analysis of antioxidant activity of Wuzhimaotao from different producing areas
Objective:To explore the extraction process of general flavone of Wuzhimaotao and compare the antioxidant activity of Wuzhimaotao from different producing areas.Methods:Box-Behnken response surface method was used to opti-mize the extraction process of general flavone of Wuzhimaotao.The content and antioxidant activity of general flavone of Wu-zhimaotao from different producing areas were determined.Results:The order affecting the extraction effect of general fla-vone of Wuzhimaotao was extraction times>extraction time>ethanol volume fraction>liquid-solid ratio.The optimum extrac-tion process was as follows:extraction time was 2.1 h,extraction times was 2 times,ethanol volume fraction was 70%,and liquid-solid ratio was 20:1.The optimal extraction process was stable,efficient and feasible,and the overall evaluation value was 5.31 mg/g.The contents of general flavone of Wuzhimaotao from five producing areas,namely Yulin in Guangxi,Heyuan in Guangdong,Longhai in Fujian,Pinghe in Fujian and Nanjing in Fujian,were 6.73 mg/g,5.28 mg/g,6.89 mg/g,7.96 mg/g and 6.67 mg/g,respectively,showing strong antioxidant capacity.There were differences in the scavenging rate of hydroxyl radical of extracting solution of general flavone of Wuzhimaotao from different producing areas,and the order from strong to weak was Yulin in Guangxi>Pinghe in Fujian>Nanjing in Fujian>Heyuan in Guangdong>Longhai in Fujian.The half scavenging concentration(IC50)of DPPH free radical in the extracting solution in Wuzhimaotao from different producing areas was 0.82～9.70 mg/ml.Among them,the extracting solution in Wuzhimaotao from Nanjing in Fujian had the best scav-enging effect on DPPH free radical,while the extracting solution in Wuzhimaotao from Yulin in Guangxi had the worst scav-enging effect on DPPH free radical.There were differences in the scavenging ability of ABTS+free radicals in the extracting solution of Wuzhimaotao from different producing areas.The IC50 of ABTS+radicals of the extracting solution of Wuzhimaotao from Pinghe(Fujian),Yulin(Guangxi),Longhai(Fujian),Heyuan(Guangdong)and Nanjing(Fujian)were 0.059 mg/ml,0.063 mg/ml,0.069 mg/ml,0.075 mg/ml and 0.082 mg/ml,respectively.Conclusion:This study provides a theoretical ba-sis for the quality evaluation and subsequent resource development of Wuzhimaotao from different producing areas.
万方数据
维普
