Aspirin attenuates myocardial fibrosis in mice with ischemic heart disease
Objective To investigate the effect of aspirin on the myocardial fibrosis in mice with ischemic heart disease induced by left anterior descending ligation.Methods A total of 24 C57BL/6 mice underwent left anterior descending ligation.At 4 weeks after surgery,the mice were randomly divided into control group and aspirin group,with 12 mice in each group.Intragastric administration of aspirin(20 mg/kg)and normal saline were given daily for 4 weeks in the aspirin group and control group,respectively.Cardiac function(left ventricular ejection fraction[LVEF]and left ventricular end-diastolic diameter[LVEDd])and fibrosis(relative scar length and proportion of fibrosis)were assessed by echocardiography and Masson staining at 4 and 8 weeks postoperatively.Western blot was used to detect the protein levels of cyclooxygenase 1(Cox-1),α-smooth muscle actin(α-SMA)and α1-collagen Ⅰ(Col1α1)in myocardial tissues at 8 weeks after surgery.Results There was no significant difference in the LVEF or LVEDd at 4 weeks after surgery between the two groups(both P＞0.05).At 8 weeks after surgery,a higher level of LVEF(P=0.001 5)and a lower level of LVEDd(P=0.000 1)were reported.There was no significant difference in the relative scar length or proportion of fibrosis at 4 weeks after surgery between the two groups(both P＞0.05).At 8 weeks after surgery,smaller scar length and lower proportion of fibrosis were also reported(both P＜0.01).Meanwhile,the protein expression levels of COX-1,α-SMA and Col1 α1 in myocardial tissues treated with aspirin were significantly decreased(all P＜0.05).Conclusion Aspirin can improve cardiac function and attenuate fibrosis in mice with ischemic heart disease.
万方数据
维普
