Objective To investigate the effect of aspirin on the myocardial fibrosis in mice with ischemic heart disease induced by left anterior descending ligation.Methods A total of 24 C57BL/6 mice underwent left anterior descending ligation.At 4 weeks after surgery,the mice were randomly divided into control group and aspirin group,with 12 mice in each group.Intragastric administration of aspirin(20 mg/kg)and normal saline were given daily for 4 weeks in the aspirin group and control group,respectively.Cardiac function(left ventricular ejection fraction[LVEF]and left ventricular end-diastolic diameter[LVEDd])and fibrosis(relative scar length and proportion of fibrosis)were assessed by echocardiography and Masson staining at 4 and 8 weeks postoperatively.Western blot was used to detect the protein levels of cyclooxygenase 1(Cox-1),α-smooth muscle actin(α-SMA)and α1-collagen Ⅰ(Col1α1)in myocardial tissues at 8 weeks after surgery.Results There was no significant difference in the LVEF or LVEDd at 4 weeks after surgery between the two groups(both P＞0.05).At 8 weeks after surgery,a higher level of LVEF(P=0.001 5)and a lower level of LVEDd(P=0.000 1)were reported.There was no significant difference in the relative scar length or proportion of fibrosis at 4 weeks after surgery between the two groups(both P＞0.05).At 8 weeks after surgery,smaller scar length and lower proportion of fibrosis were also reported(both P＜0.01).Meanwhile,the protein expression levels of COX-1,α-SMA and Col1 α1 in myocardial tissues treated with aspirin were significantly decreased(all P＜0.05).Conclusion Aspirin can improve cardiac function and attenuate fibrosis in mice with ischemic heart disease.
维普
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